Angiogenic Role of MMP-2/9 Expressed on the Cell Surface of Early Endothelial Progenitor Cells/Myeloid Angiogenic Cells

Toshie Kanayasu-Toyoda, Takeshi Tanaka, Akiko Ishii-Watabe, Hiroko Kitagawa, Akifumi Matsuyama, Eriko Uchida, Teruhide Yamaguchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Since the introduction of angiogenic cell therapy using early endothelial progenitor cells (EPCs), myeloid angiogenic cells (MACs) have been expected to be useful in treating ischemic diseases. In order to elucidate the angiogenic properties of MACs/EPCs, we clarified the characteristics of MACs as compared to M2 macrophages (Mϕ{symbol}s). Comparison of the gene expression profiles of MACs and late EPCs revealed that MACs expressed greater amounts of metalloproteinase (MMP)-9. It should be noted that the profile of MMP-2/9 expression on the cell surface of MACs was similar to that of M2 Mϕ{symbol}s, and that cell surface MMP-2/9 might be an active form based on molecular size. In addition, the invasion of MACs was prohibited not only by MMP-2/9 inhibitor, but also by the hyaluronidase treatment that caused the down-regulation of MMP-9 on the cell surface of MACs and inhibited their invasion activity. These results indicate that cell surface MMP-2/9 plays an important role in the high invasion ability of MACs. The conditioned medium of both MACs and M2 Mϕ{symbol}s stimulated tube formation of endothelial cells in vitro. MACs caused an increase in vessel formation in in vivo models through the production of IL-8. We propose that the role of MACs with cell surfaces expressing MMP-2/9 is rapidly invading ischemic tissue.

Original languageEnglish
Pages (from-to)2763-2775
Number of pages13
JournalJournal of Cellular Physiology
Volume230
Issue number11
DOIs
Publication statusPublished - 01-11-2015

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Endothelial cells
Myeloid Cells
Matrix Metalloproteinases
Hyaluronoglucosaminidase
Macrophages
Matrix Metalloproteinase 9
Conditioned Culture Medium
Interleukin-8
Gene expression
Endothelial Progenitor Cells
Tissue
Cell- and Tissue-Based Therapy
Transcriptome
Down-Regulation
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kanayasu-Toyoda, Toshie ; Tanaka, Takeshi ; Ishii-Watabe, Akiko ; Kitagawa, Hiroko ; Matsuyama, Akifumi ; Uchida, Eriko ; Yamaguchi, Teruhide. / Angiogenic Role of MMP-2/9 Expressed on the Cell Surface of Early Endothelial Progenitor Cells/Myeloid Angiogenic Cells. In: Journal of Cellular Physiology. 2015 ; Vol. 230, No. 11. pp. 2763-2775.
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Kanayasu-Toyoda, T, Tanaka, T, Ishii-Watabe, A, Kitagawa, H, Matsuyama, A, Uchida, E & Yamaguchi, T 2015, 'Angiogenic Role of MMP-2/9 Expressed on the Cell Surface of Early Endothelial Progenitor Cells/Myeloid Angiogenic Cells', Journal of Cellular Physiology, vol. 230, no. 11, pp. 2763-2775. https://doi.org/10.1002/jcp.25002

Angiogenic Role of MMP-2/9 Expressed on the Cell Surface of Early Endothelial Progenitor Cells/Myeloid Angiogenic Cells. / Kanayasu-Toyoda, Toshie; Tanaka, Takeshi; Ishii-Watabe, Akiko; Kitagawa, Hiroko; Matsuyama, Akifumi; Uchida, Eriko; Yamaguchi, Teruhide.

In: Journal of Cellular Physiology, Vol. 230, No. 11, 01.11.2015, p. 2763-2775.

Research output: Contribution to journalArticle

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AU - Kanayasu-Toyoda, Toshie

AU - Tanaka, Takeshi

AU - Ishii-Watabe, Akiko

AU - Kitagawa, Hiroko

AU - Matsuyama, Akifumi

AU - Uchida, Eriko

AU - Yamaguchi, Teruhide

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