Anion Channel Blockers Inhibit the Acrosome Reaction of Echinoderm Sperm: anion channel blocker/acrosome reaction/acid release/stafish/sea urchin

ICHIRO NISHIYAMA; HAJIME SASAKI; TAEI MATSUI; MOTONORI HOSHI

doi:10.1111/j.1440-169X.1985.00461.x

Anion Channel Blockers Inhibit the Acrosome Reaction of Echinoderm Sperm: anion channel blocker/acrosome reaction/acid release/stafish/sea urchin

ICHIRO NISHIYAMA
, HAJIME SASAKI
, TAEI MATSUI
, MOTONORI HOSHI

Faculty of Medical Technology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Two types of anion channel blockers, SITS (4‐acetamide‐4‘‐isothiocyanostilbene‐2,2′‐disulfonic acid) and DIDS (4,4′‐diisothiocyanostilbene‐2, 2’‐disulfonic acid), inhibited jelly‐induced acrosorne reaction in starfish and sea urchin. In starfish sperm, both of the blockers reversibly inhibited the formation of acrosomal process but they had no effect on either the acrosomal exocytosis or acid release from the sperm. Complete acrosome reaction occurred even in Cl⁻‐ and SO₄²⁻‐free artificial seawater whereas HCO₃⁻was required for the acrosomal exocytosis. Importance of anion transport in acrosome reaction is discussed.

Original language	English
Pages (from-to)	461-468
Number of pages	8
Journal	Development, Growth & Differentiation
Volume	27
Issue number	4
DOIs	https://doi.org/10.1111/j.1440-169X.1985.00461.x
Publication status	Published - 1985

All Science Journal Classification (ASJC) codes

Developmental Biology
Cell Biology

Access to Document

10.1111/j.1440-169X.1985.00461.x

Cite this

@article{ea2a9d7e0cb44bb4a10dda51508ecc46,

title = "Anion Channel Blockers Inhibit the Acrosome Reaction of Echinoderm Sperm: anion channel blocker/acrosome reaction/acid release/stafish/sea urchin",

abstract = "Two types of anion channel blockers, SITS (4‐acetamide‐4{\textquoteleft}‐isothiocyanostilbene‐2,2′‐disulfonic acid) and DIDS (4,4′‐diisothiocyanostilbene‐2, 2{\textquoteright}‐disulfonic acid), inhibited jelly‐induced acrosorne reaction in starfish and sea urchin. In starfish sperm, both of the blockers reversibly inhibited the formation of acrosomal process but they had no effect on either the acrosomal exocytosis or acid release from the sperm. Complete acrosome reaction occurred even in Cl−‐ and SO42−‐free artificial seawater whereas HCO3−was required for the acrosomal exocytosis. Importance of anion transport in acrosome reaction is discussed.",

author = "ICHIRO NISHIYAMA and HAJIME SASAKI and TAEI MATSUI and MOTONORI HOSHI",

year = "1985",

doi = "10.1111/j.1440-169X.1985.00461.x",

language = "English",

volume = "27",

pages = "461--468",

journal = "Development, Growth \& Differentiation",

issn = "0012-1592",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "4",

}

TY - JOUR

T1 - Anion Channel Blockers Inhibit the Acrosome Reaction of Echinoderm Sperm

T2 - anion channel blocker/acrosome reaction/acid release/stafish/sea urchin

AU - NISHIYAMA, ICHIRO

AU - SASAKI, HAJIME

AU - MATSUI, TAEI

AU - HOSHI, MOTONORI

PY - 1985

Y1 - 1985

N2 - Two types of anion channel blockers, SITS (4‐acetamide‐4‘‐isothiocyanostilbene‐2,2′‐disulfonic acid) and DIDS (4,4′‐diisothiocyanostilbene‐2, 2’‐disulfonic acid), inhibited jelly‐induced acrosorne reaction in starfish and sea urchin. In starfish sperm, both of the blockers reversibly inhibited the formation of acrosomal process but they had no effect on either the acrosomal exocytosis or acid release from the sperm. Complete acrosome reaction occurred even in Cl−‐ and SO42−‐free artificial seawater whereas HCO3−was required for the acrosomal exocytosis. Importance of anion transport in acrosome reaction is discussed.

AB - Two types of anion channel blockers, SITS (4‐acetamide‐4‘‐isothiocyanostilbene‐2,2′‐disulfonic acid) and DIDS (4,4′‐diisothiocyanostilbene‐2, 2’‐disulfonic acid), inhibited jelly‐induced acrosorne reaction in starfish and sea urchin. In starfish sperm, both of the blockers reversibly inhibited the formation of acrosomal process but they had no effect on either the acrosomal exocytosis or acid release from the sperm. Complete acrosome reaction occurred even in Cl−‐ and SO42−‐free artificial seawater whereas HCO3−was required for the acrosomal exocytosis. Importance of anion transport in acrosome reaction is discussed.

UR - https://www.scopus.com/pages/publications/0022387605

UR - https://www.scopus.com/pages/publications/0022387605#tab=citedBy

U2 - 10.1111/j.1440-169X.1985.00461.x

DO - 10.1111/j.1440-169X.1985.00461.x

M3 - Article

AN - SCOPUS:0022387605

SN - 0012-1592

VL - 27

SP - 461

EP - 468

JO - Development, Growth & Differentiation

JF - Development, Growth & Differentiation

IS - 4

ER -

Anion Channel Blockers Inhibit the Acrosome Reaction of Echinoderm Sperm: anion channel blocker/acrosome reaction/acid release/stafish/sea urchin

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this