Anopheles dirus co-infection with human and monkey malaria parasites in Vietnam

Shusuke Nakazawa, Ron P. Marchand, Nguyen Tuyen Quang, Richard Culleton, Nguyen Duc Manh, Yoshimasa Maeno

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The feasibility of identifying parasite DNA and specific mRNAs from wild-caught Anopheles dirus mosquitoes was assessed using dried mosquito salivary glands preserved on filter paper. We were able to detect Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium knowlesi DNA by conventional PCR and, furthermore, detected P. falciparum gametocyte-specific genes, pfg377 and pfs16 mRNA, P. knowlesi circumsporozoite protein (CSP) and sporozoite surface protein 2 (SSP2) mRNA by reverse transcription-PCR. Using this technique, we were able to confirm the presence of P. vivax, P. falciparum and P. knowlesi in one particular wild-caught mosquito. These results indicate that P. knowlesi may be transmitted by the primary human malaria vector in forested areas in Vietnam. This study also shows that the preservation of mosquito salivary glands on filter paper, and the down-stream extraction of parasite DNA and RNA from those, offers a powerful resource for molecular epidemiological studies on malaria.

Original languageEnglish
Pages (from-to)1533-1537
Number of pages5
JournalInternational Journal for Parasitology
Volume39
Issue number14
DOIs
Publication statusPublished - 01-12-2009

Fingerprint

Anopheles
Plasmodium knowlesi
Vietnam
Culicidae
Coinfection
Malaria
Haplorhini
Parasites
Plasmodium falciparum
Salivary Glands
Messenger RNA
DNA
Plasmodium malariae
Vivax Malaria
Plasmodium vivax
Sporozoites
Polymerase Chain Reaction
Reverse Transcription
Epidemiologic Studies
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Nakazawa, Shusuke ; Marchand, Ron P. ; Quang, Nguyen Tuyen ; Culleton, Richard ; Manh, Nguyen Duc ; Maeno, Yoshimasa. / Anopheles dirus co-infection with human and monkey malaria parasites in Vietnam. In: International Journal for Parasitology. 2009 ; Vol. 39, No. 14. pp. 1533-1537.
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Anopheles dirus co-infection with human and monkey malaria parasites in Vietnam. / Nakazawa, Shusuke; Marchand, Ron P.; Quang, Nguyen Tuyen; Culleton, Richard; Manh, Nguyen Duc; Maeno, Yoshimasa.

In: International Journal for Parasitology, Vol. 39, No. 14, 01.12.2009, p. 1533-1537.

Research output: Contribution to journalArticle

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