TY - JOUR
T1 - Anopheles dirus co-infection with human and monkey malaria parasites in Vietnam
AU - Nakazawa, Shusuke
AU - Marchand, Ron P.
AU - Quang, Nguyen Tuyen
AU - Culleton, Richard
AU - Manh, Nguyen Duc
AU - Maeno, Yoshimasa
N1 - Funding Information:
We thank Dr. Le Duc Dao for his technical advice. This study was partly supported by a Core University Program of the Japan Society for the Promotion of Science and a Grant-in-Aid for the Japan-Vietnam Collaborative Research Program from the Japan Society for the Promotion of Science. The Khanh Phu Malaria Research team is financed by the Medical Committee Netherlands-Vietnam.
PY - 2009/12
Y1 - 2009/12
N2 - The feasibility of identifying parasite DNA and specific mRNAs from wild-caught Anopheles dirus mosquitoes was assessed using dried mosquito salivary glands preserved on filter paper. We were able to detect Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium knowlesi DNA by conventional PCR and, furthermore, detected P. falciparum gametocyte-specific genes, pfg377 and pfs16 mRNA, P. knowlesi circumsporozoite protein (CSP) and sporozoite surface protein 2 (SSP2) mRNA by reverse transcription-PCR. Using this technique, we were able to confirm the presence of P. vivax, P. falciparum and P. knowlesi in one particular wild-caught mosquito. These results indicate that P. knowlesi may be transmitted by the primary human malaria vector in forested areas in Vietnam. This study also shows that the preservation of mosquito salivary glands on filter paper, and the down-stream extraction of parasite DNA and RNA from those, offers a powerful resource for molecular epidemiological studies on malaria.
AB - The feasibility of identifying parasite DNA and specific mRNAs from wild-caught Anopheles dirus mosquitoes was assessed using dried mosquito salivary glands preserved on filter paper. We were able to detect Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium knowlesi DNA by conventional PCR and, furthermore, detected P. falciparum gametocyte-specific genes, pfg377 and pfs16 mRNA, P. knowlesi circumsporozoite protein (CSP) and sporozoite surface protein 2 (SSP2) mRNA by reverse transcription-PCR. Using this technique, we were able to confirm the presence of P. vivax, P. falciparum and P. knowlesi in one particular wild-caught mosquito. These results indicate that P. knowlesi may be transmitted by the primary human malaria vector in forested areas in Vietnam. This study also shows that the preservation of mosquito salivary glands on filter paper, and the down-stream extraction of parasite DNA and RNA from those, offers a powerful resource for molecular epidemiological studies on malaria.
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U2 - 10.1016/j.ijpara.2009.08.005
DO - 10.1016/j.ijpara.2009.08.005
M3 - Article
C2 - 19703460
AN - SCOPUS:70349971895
SN - 0020-7519
VL - 39
SP - 1533
EP - 1537
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 14
ER -
