Anti-calreticulin antibody binds to a membrane protein in caveolae

Ryuji Nomura, Takeo Aoki, Haruo Hagiwara, Takao Senda, Toyoshi Fujimoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Caveolae have been reported to contain a number of proteins related to Ca2+ mobilization, including an inositol 1,4,5-trisphosphate receptor-like protein, and a plasmalemmal calcium pump. In search of other Ca2+-related proteins, we found that a polyclonal antibody to calreticulin (CRT), an endoplasmic reticulum (ER) Ca2+-binding protein, binds to the cytoplasmic surface of caveolae. By immunofluorescence microscopy of cultured fibroblasts, labeling by the anti-CRT antibody was found colocalized with caveolin-1. Immunogold electron microscopy of ultrathin frozen sections showed that the antibody decorates the cytoplasmic surface of caveolae. The caveolar labeling could be due to CRT that leaked out of the ER, but the labeling persisted even when isolated plasmalemmal preparations were treated with 10 mM EDTA, 1 M NaCl, or 0.1 M Na2CO3 (pH 11). Furthermore, in SDS-digested freeze-fracture replicas, labeling was associated with caveolae in the P face of the plasma membrane. However, in cells transfected with hemagglutinin (HA)-tagged cDNA, the labeling by anti-HA was observed only in the ER and was not found in caveolae. Taken together, the results suggest that a protein structurally similar to CRT is localized in caveolae as an integral membrane protein, and that the protein could be functionally related to the intracellular Ca2+ homeostasis.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
JournalActa Histochemica et Cytochemica
Volume38
Issue number1
DOIs
Publication statusPublished - 06-05-2005

Fingerprint

Calreticulin
Caveolae
Labeling
Anti-Idiotypic Antibodies
Membrane Proteins
Antibodies
Endoplasmic Reticulum
Hemagglutinins
Proteins
Inositol 1,4,5-Trisphosphate Receptors
Caveolin 1
Fibroblasts
Cell membranes
Edetic Acid
Electron microscopy
Frozen Sections
Carrier Proteins
Microscopic examination
Fluorescence Microscopy
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

Cite this

Nomura, Ryuji ; Aoki, Takeo ; Hagiwara, Haruo ; Senda, Takao ; Fujimoto, Toyoshi. / Anti-calreticulin antibody binds to a membrane protein in caveolae. In: Acta Histochemica et Cytochemica. 2005 ; Vol. 38, No. 1. pp. 43-52.
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Anti-calreticulin antibody binds to a membrane protein in caveolae. / Nomura, Ryuji; Aoki, Takeo; Hagiwara, Haruo; Senda, Takao; Fujimoto, Toyoshi.

In: Acta Histochemica et Cytochemica, Vol. 38, No. 1, 06.05.2005, p. 43-52.

Research output: Contribution to journalArticle

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