Anti-DFS70 Antibodies in 597 Healthy Hospital Workers

Akihiro Watanabe, Masanari Kodera, Kazumitsu Sugiura, Toshikazu Usuda, Eng M. Tan, Yoshinari Takasaki, Yasushi Tomita, Yoshinao Muro

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Objective. Autoantibodies against DFS70 (dense fine speckles 70) antigen (also known as tens epithelium-derived growth factor) have been recently identified among the antinuclear antibodies (ANAs) in patients with atopic disorders. We undertook this study to examine the frequency of anti-DFS70 antibodies in a large number of healthy people. Methods. Sera of 597 healthy individuals working in a hospital (142 men, 455 women) were analyzed for ANAs and for anti-DFS70 antibodies by indirect immunofluorescence (IIF) with HEp-2 cells as a substrate and by immunoblotting using DFS70 recombinant protein and whole HeLa cell extract. Results. ANAs were present in 20% of all individuals by IIF. Nine percent of subjects were ANA positive at a serum dilution of 1:40, 4.0% at 1:80, 5.5% at 1:160, 1.0% at 1:320, and 0.3% at 1:640. There were 64 anti-DFS70 antibody-positive individuals. Surprisingly, this was 11% of the whole population and 54% of the ANA-positive population. The percentage of female anti-DFS70 antibody-positive subjects (86%; 55 of 64 subjects) was higher than the percentage of female anti-DFS70 antibody-negative subjects (75%; 398 of 533 subjects) (P < 0.05). The prevalence of anti-DFS70 antibody-positive sera decreased with increasing age (P = 0.0017). Conclusion. Considering that anti-DF870 antibody positivity is rare in patients with systemic autoimmune diseases, introducing the anti-DFS70 antibody examination as a screening test for ANA-positive persons could be used to rule out systemic autoimmune diseases, resulting in considerable cost-saving potential. In addition, this test defines a subpopulation of healthy people in whom long-term followup might reveal health-related implications of this finding, since anti-DFS70 antibodies have been shown to be associated with some illnesses.

Original languageEnglish
Pages (from-to)892-900
Number of pages9
JournalArthritis and Rheumatism
Volume50
Issue number3
DOIs
Publication statusPublished - 01-03-2004

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Antinuclear Antibodies
Antibodies
Indirect Fluorescent Antibody Technique
Autoimmune Diseases
Serum
Cell Extracts
HeLa Cells
Recombinant Proteins
Immunoblotting
Autoantibodies
Population
Anti-Idiotypic Antibodies
Intercellular Signaling Peptides and Proteins
Epithelium
Antigens
Costs and Cost Analysis
Health

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Watanabe, A., Kodera, M., Sugiura, K., Usuda, T., Tan, E. M., Takasaki, Y., ... Muro, Y. (2004). Anti-DFS70 Antibodies in 597 Healthy Hospital Workers. Arthritis and Rheumatism, 50(3), 892-900. https://doi.org/10.1002/art.20096
Watanabe, Akihiro ; Kodera, Masanari ; Sugiura, Kazumitsu ; Usuda, Toshikazu ; Tan, Eng M. ; Takasaki, Yoshinari ; Tomita, Yasushi ; Muro, Yoshinao. / Anti-DFS70 Antibodies in 597 Healthy Hospital Workers. In: Arthritis and Rheumatism. 2004 ; Vol. 50, No. 3. pp. 892-900.
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Watanabe, A, Kodera, M, Sugiura, K, Usuda, T, Tan, EM, Takasaki, Y, Tomita, Y & Muro, Y 2004, 'Anti-DFS70 Antibodies in 597 Healthy Hospital Workers', Arthritis and Rheumatism, vol. 50, no. 3, pp. 892-900. https://doi.org/10.1002/art.20096

Anti-DFS70 Antibodies in 597 Healthy Hospital Workers. / Watanabe, Akihiro; Kodera, Masanari; Sugiura, Kazumitsu; Usuda, Toshikazu; Tan, Eng M.; Takasaki, Yoshinari; Tomita, Yasushi; Muro, Yoshinao.

In: Arthritis and Rheumatism, Vol. 50, No. 3, 01.03.2004, p. 892-900.

Research output: Contribution to journalArticle

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T1 - Anti-DFS70 Antibodies in 597 Healthy Hospital Workers

AU - Watanabe, Akihiro

AU - Kodera, Masanari

AU - Sugiura, Kazumitsu

AU - Usuda, Toshikazu

AU - Tan, Eng M.

AU - Takasaki, Yoshinari

AU - Tomita, Yasushi

AU - Muro, Yoshinao

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N2 - Objective. Autoantibodies against DFS70 (dense fine speckles 70) antigen (also known as tens epithelium-derived growth factor) have been recently identified among the antinuclear antibodies (ANAs) in patients with atopic disorders. We undertook this study to examine the frequency of anti-DFS70 antibodies in a large number of healthy people. Methods. Sera of 597 healthy individuals working in a hospital (142 men, 455 women) were analyzed for ANAs and for anti-DFS70 antibodies by indirect immunofluorescence (IIF) with HEp-2 cells as a substrate and by immunoblotting using DFS70 recombinant protein and whole HeLa cell extract. Results. ANAs were present in 20% of all individuals by IIF. Nine percent of subjects were ANA positive at a serum dilution of 1:40, 4.0% at 1:80, 5.5% at 1:160, 1.0% at 1:320, and 0.3% at 1:640. There were 64 anti-DFS70 antibody-positive individuals. Surprisingly, this was 11% of the whole population and 54% of the ANA-positive population. The percentage of female anti-DFS70 antibody-positive subjects (86%; 55 of 64 subjects) was higher than the percentage of female anti-DFS70 antibody-negative subjects (75%; 398 of 533 subjects) (P < 0.05). The prevalence of anti-DFS70 antibody-positive sera decreased with increasing age (P = 0.0017). Conclusion. Considering that anti-DF870 antibody positivity is rare in patients with systemic autoimmune diseases, introducing the anti-DFS70 antibody examination as a screening test for ANA-positive persons could be used to rule out systemic autoimmune diseases, resulting in considerable cost-saving potential. In addition, this test defines a subpopulation of healthy people in whom long-term followup might reveal health-related implications of this finding, since anti-DFS70 antibodies have been shown to be associated with some illnesses.

AB - Objective. Autoantibodies against DFS70 (dense fine speckles 70) antigen (also known as tens epithelium-derived growth factor) have been recently identified among the antinuclear antibodies (ANAs) in patients with atopic disorders. We undertook this study to examine the frequency of anti-DFS70 antibodies in a large number of healthy people. Methods. Sera of 597 healthy individuals working in a hospital (142 men, 455 women) were analyzed for ANAs and for anti-DFS70 antibodies by indirect immunofluorescence (IIF) with HEp-2 cells as a substrate and by immunoblotting using DFS70 recombinant protein and whole HeLa cell extract. Results. ANAs were present in 20% of all individuals by IIF. Nine percent of subjects were ANA positive at a serum dilution of 1:40, 4.0% at 1:80, 5.5% at 1:160, 1.0% at 1:320, and 0.3% at 1:640. There were 64 anti-DFS70 antibody-positive individuals. Surprisingly, this was 11% of the whole population and 54% of the ANA-positive population. The percentage of female anti-DFS70 antibody-positive subjects (86%; 55 of 64 subjects) was higher than the percentage of female anti-DFS70 antibody-negative subjects (75%; 398 of 533 subjects) (P < 0.05). The prevalence of anti-DFS70 antibody-positive sera decreased with increasing age (P = 0.0017). Conclusion. Considering that anti-DF870 antibody positivity is rare in patients with systemic autoimmune diseases, introducing the anti-DFS70 antibody examination as a screening test for ANA-positive persons could be used to rule out systemic autoimmune diseases, resulting in considerable cost-saving potential. In addition, this test defines a subpopulation of healthy people in whom long-term followup might reveal health-related implications of this finding, since anti-DFS70 antibodies have been shown to be associated with some illnesses.

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Watanabe A, Kodera M, Sugiura K, Usuda T, Tan EM, Takasaki Y et al. Anti-DFS70 Antibodies in 597 Healthy Hospital Workers. Arthritis and Rheumatism. 2004 Mar 1;50(3):892-900. https://doi.org/10.1002/art.20096