Anti-DFS70 Antibodies in 597 Healthy Hospital Workers

Akihiro Watanabe, Masanari Kodera, Kazumitsu Sugiura, Toshikazu Usuda, Eng M. Tan, Yoshinari Takasaki, Yasushi Tomita, Yoshinao Muro

Research output: Contribution to journalArticlepeer-review

130 Citations (Scopus)

Abstract

Objective. Autoantibodies against DFS70 (dense fine speckles 70) antigen (also known as tens epithelium-derived growth factor) have been recently identified among the antinuclear antibodies (ANAs) in patients with atopic disorders. We undertook this study to examine the frequency of anti-DFS70 antibodies in a large number of healthy people. Methods. Sera of 597 healthy individuals working in a hospital (142 men, 455 women) were analyzed for ANAs and for anti-DFS70 antibodies by indirect immunofluorescence (IIF) with HEp-2 cells as a substrate and by immunoblotting using DFS70 recombinant protein and whole HeLa cell extract. Results. ANAs were present in 20% of all individuals by IIF. Nine percent of subjects were ANA positive at a serum dilution of 1:40, 4.0% at 1:80, 5.5% at 1:160, 1.0% at 1:320, and 0.3% at 1:640. There were 64 anti-DFS70 antibody-positive individuals. Surprisingly, this was 11% of the whole population and 54% of the ANA-positive population. The percentage of female anti-DFS70 antibody-positive subjects (86%; 55 of 64 subjects) was higher than the percentage of female anti-DFS70 antibody-negative subjects (75%; 398 of 533 subjects) (P < 0.05). The prevalence of anti-DFS70 antibody-positive sera decreased with increasing age (P = 0.0017). Conclusion. Considering that anti-DF870 antibody positivity is rare in patients with systemic autoimmune diseases, introducing the anti-DFS70 antibody examination as a screening test for ANA-positive persons could be used to rule out systemic autoimmune diseases, resulting in considerable cost-saving potential. In addition, this test defines a subpopulation of healthy people in whom long-term followup might reveal health-related implications of this finding, since anti-DFS70 antibodies have been shown to be associated with some illnesses.

Original languageEnglish
Pages (from-to)892-900
Number of pages9
JournalArthritis and Rheumatism
Volume50
Issue number3
DOIs
Publication statusPublished - 03-2004

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Anti-DFS70 Antibodies in 597 Healthy Hospital Workers'. Together they form a unique fingerprint.

Cite this