Anti-GM1 antibodies affect the integrity of lipid rafts

Akihiro Ueda, Sayuri Shima, Tadayuki Miyashita, Shinji Ito, Masami Ueda, Susumu Kusunoki, Kunihiko Asakura, Tatsuro Mutoh

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


Previous studies have shown that patients with the axonal form of Guillain-Barré syndrome (GBS) develop autoantibodies against GM1 ganglioside (GM1). Nerve growth factor (NGF) is essential for neuronal survival in vivo and its functional receptor is Trk-tyrosine kinase. Here, we examined the biological effects of sera from patients with the axonal form of GBS on the morphology and the phosphorylation state of Trk-tyrosine kinase in PC12 cells. Furthermore, we examined the effect of the sera on the integrity of membrane lipid rafts biochemically. The data show that anti-GM1 antibodies found in patients' sera but not control sera inhibit NGF-induced Trk autophosphorylation. Most intriguingly, the autoantibodies alter the distribution of Trk in lipid rafts without shifting the distribution of a rafts marker protein. These data strongly suggest that anti-GM1 antibodies directly influence the integrity of the signaling platform, lipid rafts, implicating the importance of lipid rafts in the development of this disorder.

Original languageEnglish
Pages (from-to)355-362
Number of pages8
JournalMolecular and Cellular Neuroscience
Issue number4
Publication statusPublished - 12-2010

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology


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