Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells

Eiji Munetsuna, Rie Kawanami, Miyu Nishikawa, Shinnosuke Ikeda, Sachie Nakabayashi, Kaori Yasuda, Miho Ohta, Masaki Kamakura, Shinichi Ikushiro, Toshiyuki Sakaki

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

1α-Hydroxylation of 25-hydroxyvitamin D3 is believed to be essential for its biological effects. In this study, we evaluated the biological activity of 25(OH)D3 itself comparing with the effect of cell-derived 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3). First, we measured the cell-derived 1α,25(OH)2D3 level in immortalized human prostate cell (PZ-HPV-7) using [3H]-25(OH)D3. The effects of the cell-derived 1α,25(OH)2D3 on vitamin D3 24-hydroxylase (CYP24A1) mRNA level and the cell growth inhibition were significantly lower than the effects of 25(OH)D3 itself added to cell culture. 25-Hydroxyvitamin D3 1α-hydroxylase (CYP27B1) gene knockdown had no significant effects on the 25(OH)D3-dependent effects, whereas vitamin D receptor (VDR) gene knockdown resulted in a significant decrease in the 25(OH)D3-dependent effects. These results strongly suggest that 25(OH)D3 can directly bind to VDR and exerts its biological functions. DNA microarray and real-time RT-PCR analyses suggest that semaphorin 3B, cystatin E/M, and cystatin D may be involved in the antiproliferative effect of 25(OH)D3.

Original languageEnglish
Pages (from-to)960-970
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume382
Issue number2
DOIs
Publication statusPublished - 15-02-2014

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Cystatin M
Calcifediol
Calcitriol Receptors
Prostate
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Genes
Semaphorins
Hydroxylation
Calcitriol
Gene Knockdown Techniques
Cell growth
Microarrays
Mixed Function Oxygenases
Bioactivity
Cell culture
Messenger RNA
Cystatins
DNA
Oligonucleotide Array Sequence Analysis
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Munetsuna, E., Kawanami, R., Nishikawa, M., Ikeda, S., Nakabayashi, S., Yasuda, K., ... Sakaki, T. (2014). Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells. Molecular and Cellular Endocrinology, 382(2), 960-970. https://doi.org/10.1016/j.mce.2013.11.014
Munetsuna, Eiji ; Kawanami, Rie ; Nishikawa, Miyu ; Ikeda, Shinnosuke ; Nakabayashi, Sachie ; Yasuda, Kaori ; Ohta, Miho ; Kamakura, Masaki ; Ikushiro, Shinichi ; Sakaki, Toshiyuki. / Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells. In: Molecular and Cellular Endocrinology. 2014 ; Vol. 382, No. 2. pp. 960-970.
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Munetsuna, E, Kawanami, R, Nishikawa, M, Ikeda, S, Nakabayashi, S, Yasuda, K, Ohta, M, Kamakura, M, Ikushiro, S & Sakaki, T 2014, 'Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells', Molecular and Cellular Endocrinology, vol. 382, no. 2, pp. 960-970. https://doi.org/10.1016/j.mce.2013.11.014

Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells. / Munetsuna, Eiji; Kawanami, Rie; Nishikawa, Miyu; Ikeda, Shinnosuke; Nakabayashi, Sachie; Yasuda, Kaori; Ohta, Miho; Kamakura, Masaki; Ikushiro, Shinichi; Sakaki, Toshiyuki.

In: Molecular and Cellular Endocrinology, Vol. 382, No. 2, 15.02.2014, p. 960-970.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells

AU - Munetsuna, Eiji

AU - Kawanami, Rie

AU - Nishikawa, Miyu

AU - Ikeda, Shinnosuke

AU - Nakabayashi, Sachie

AU - Yasuda, Kaori

AU - Ohta, Miho

AU - Kamakura, Masaki

AU - Ikushiro, Shinichi

AU - Sakaki, Toshiyuki

PY - 2014/2/15

Y1 - 2014/2/15

N2 - 1α-Hydroxylation of 25-hydroxyvitamin D3 is believed to be essential for its biological effects. In this study, we evaluated the biological activity of 25(OH)D3 itself comparing with the effect of cell-derived 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3). First, we measured the cell-derived 1α,25(OH)2D3 level in immortalized human prostate cell (PZ-HPV-7) using [3H]-25(OH)D3. The effects of the cell-derived 1α,25(OH)2D3 on vitamin D3 24-hydroxylase (CYP24A1) mRNA level and the cell growth inhibition were significantly lower than the effects of 25(OH)D3 itself added to cell culture. 25-Hydroxyvitamin D3 1α-hydroxylase (CYP27B1) gene knockdown had no significant effects on the 25(OH)D3-dependent effects, whereas vitamin D receptor (VDR) gene knockdown resulted in a significant decrease in the 25(OH)D3-dependent effects. These results strongly suggest that 25(OH)D3 can directly bind to VDR and exerts its biological functions. DNA microarray and real-time RT-PCR analyses suggest that semaphorin 3B, cystatin E/M, and cystatin D may be involved in the antiproliferative effect of 25(OH)D3.

AB - 1α-Hydroxylation of 25-hydroxyvitamin D3 is believed to be essential for its biological effects. In this study, we evaluated the biological activity of 25(OH)D3 itself comparing with the effect of cell-derived 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3). First, we measured the cell-derived 1α,25(OH)2D3 level in immortalized human prostate cell (PZ-HPV-7) using [3H]-25(OH)D3. The effects of the cell-derived 1α,25(OH)2D3 on vitamin D3 24-hydroxylase (CYP24A1) mRNA level and the cell growth inhibition were significantly lower than the effects of 25(OH)D3 itself added to cell culture. 25-Hydroxyvitamin D3 1α-hydroxylase (CYP27B1) gene knockdown had no significant effects on the 25(OH)D3-dependent effects, whereas vitamin D receptor (VDR) gene knockdown resulted in a significant decrease in the 25(OH)D3-dependent effects. These results strongly suggest that 25(OH)D3 can directly bind to VDR and exerts its biological functions. DNA microarray and real-time RT-PCR analyses suggest that semaphorin 3B, cystatin E/M, and cystatin D may be involved in the antiproliferative effect of 25(OH)D3.

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