Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells

Eiji Munetsuna, Rie Kawanami, Miyu Nishikawa, Shinnosuke Ikeda, Sachie Nakabayashi, Kaori Yasuda, Miho Ohta, Masaki Kamakura, Shinichi Ikushiro, Toshiyuki Sakaki

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Abstract

1α-Hydroxylation of 25-hydroxyvitamin D3 is believed to be essential for its biological effects. In this study, we evaluated the biological activity of 25(OH)D3 itself comparing with the effect of cell-derived 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3). First, we measured the cell-derived 1α,25(OH)2D3 level in immortalized human prostate cell (PZ-HPV-7) using [3H]-25(OH)D3. The effects of the cell-derived 1α,25(OH)2D3 on vitamin D3 24-hydroxylase (CYP24A1) mRNA level and the cell growth inhibition were significantly lower than the effects of 25(OH)D3 itself added to cell culture. 25-Hydroxyvitamin D3 1α-hydroxylase (CYP27B1) gene knockdown had no significant effects on the 25(OH)D3-dependent effects, whereas vitamin D receptor (VDR) gene knockdown resulted in a significant decrease in the 25(OH)D3-dependent effects. These results strongly suggest that 25(OH)D3 can directly bind to VDR and exerts its biological functions. DNA microarray and real-time RT-PCR analyses suggest that semaphorin 3B, cystatin E/M, and cystatin D may be involved in the antiproliferative effect of 25(OH)D3.

Original languageEnglish
Pages (from-to)960-970
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume382
Issue number2
DOIs
Publication statusPublished - 15-02-2014

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Munetsuna, E., Kawanami, R., Nishikawa, M., Ikeda, S., Nakabayashi, S., Yasuda, K., Ohta, M., Kamakura, M., Ikushiro, S., & Sakaki, T. (2014). Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells. Molecular and Cellular Endocrinology, 382(2), 960-970. https://doi.org/10.1016/j.mce.2013.11.014