Antibody fusions with fluorescent proteins: A versatile reagent for profiling protein expression

Kazuhiko Morino, Harue Katsumi, Yasushi Akahori, Yoshitaka Iba, Midori Shinohara, Yoshinori Ukai, Yuji Kohara, Yoshikazu Kurosawa

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

We developed a system by which antibodies, fused to fluorescent proteins with different wavelengths, can be prepared within a month against various antigens. An antibody library composed of a large number of single-chain Fv-CL fragment was constructed by means of a phage-display system. The constructs were designed to facilitate changing of the protein forms by simple enzyme manipulation. In the present study, we adopted a molecular form of antibody in which a single-chain Fv-CL fragment is fused with a green fluorescent protein (GFP) or red fluorescent protein (RFP). In addition, a His-tag was inserted between CL and GFP (or RFP). We describe the utility of this system using Caenorhabditis elegans embryo as a model.

Original languageEnglish
Pages (from-to)175-184
Number of pages10
JournalJournal of immunological methods
Volume257
Issue number1-2
DOIs
Publication statusPublished - 01-11-2001

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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