Antibody-mediated redistribution and shedding of endothelial antigens in the rabbit

Yukio Yuzawa, J. R. Brentjens, J. Brett, P. R.B. Caldwell, C. Esposito, A. Fukatsu, G. Godman, D. Stern, G. Andres

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We report the results of studies performed in vitro and in vivo that were designed to explore individual, sequential, and concurrent Ag-antibody interactions at the surface of rabbit endothelial cells. Divalent heterologous antibodies to rabbit lung angiotensin-converting enzyme and to rabbit lung thrombomodulin were employed. In cultured monolayers, both antibodies redistributed the specific Ag and co-redistributed the immunologically unrelated Ag inducing partial or complete disappearance of the Ag from the cell surface (antigenic modulation) in 15 to 60 min. When injected into living rabbits, each antibody induced a rapid (1 to 3 min) redistribution and subsequent modulation of the specific and of the unrelated Ag at the surface of alveolar endothelial cells. Immune complexes, and the unrelated Ag, were shed in the circulation, attaining peak levels 3 to 4 min after the injection; were rapidly bound by platelets, E, and polymorphonuclear leukocytes; and were subsequently found in phagocytic cells in the spleen and in the liver. Thrombomodulin co-shed by angiotensin- converting enzyme antibody and, to a lesser degree, angiotensin-converting enzyme co-shed by thrombomodulin antibody, crossed the glomerular capillary walls and were reabsorbed by the epithelial cells of the proximal tubules within 2 to 3 min. The results show that immunologically unrelated Ag can be passively entrapped during formation of immune complexes at the cell surface, and provide new information on the kinetics of clearance of immune complexes containing endogenous, structural Ag.

Original languageEnglish
Pages (from-to)5633-5646
Number of pages14
JournalJournal of Immunology
Volume150
Issue number12
Publication statusPublished - 01-01-1993
Externally publishedYes

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Thrombomodulin
Rabbits
Antigens
Peptidyl-Dipeptidase A
Antigen-Antibody Complex
Antibodies
Antigenic Modulation
Endothelial Cells
Heterophile Antibodies
Alveolar Epithelial Cells
Lung
Phagocytes
Neutrophils
Blood Platelets
Spleen
Epithelial Cells
Injections
Liver

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Yuzawa, Y., Brentjens, J. R., Brett, J., Caldwell, P. R. B., Esposito, C., Fukatsu, A., ... Andres, G. (1993). Antibody-mediated redistribution and shedding of endothelial antigens in the rabbit. Journal of Immunology, 150(12), 5633-5646.
Yuzawa, Yukio ; Brentjens, J. R. ; Brett, J. ; Caldwell, P. R.B. ; Esposito, C. ; Fukatsu, A. ; Godman, G. ; Stern, D. ; Andres, G. / Antibody-mediated redistribution and shedding of endothelial antigens in the rabbit. In: Journal of Immunology. 1993 ; Vol. 150, No. 12. pp. 5633-5646.
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Yuzawa, Y, Brentjens, JR, Brett, J, Caldwell, PRB, Esposito, C, Fukatsu, A, Godman, G, Stern, D & Andres, G 1993, 'Antibody-mediated redistribution and shedding of endothelial antigens in the rabbit', Journal of Immunology, vol. 150, no. 12, pp. 5633-5646.

Antibody-mediated redistribution and shedding of endothelial antigens in the rabbit. / Yuzawa, Yukio; Brentjens, J. R.; Brett, J.; Caldwell, P. R.B.; Esposito, C.; Fukatsu, A.; Godman, G.; Stern, D.; Andres, G.

In: Journal of Immunology, Vol. 150, No. 12, 01.01.1993, p. 5633-5646.

Research output: Contribution to journalArticle

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Yuzawa Y, Brentjens JR, Brett J, Caldwell PRB, Esposito C, Fukatsu A et al. Antibody-mediated redistribution and shedding of endothelial antigens in the rabbit. Journal of Immunology. 1993 Jan 1;150(12):5633-5646.