Antigenic variation in Bordetella pertussis isolates recovered from adults and children in Japan

Hyun Ja Han, Kazunari Kamachi, Kenji Okada, Hiromi Toyoizumi-Ajisaka, Yuko Sasaki, Yoshichika Arakawa

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23 Citations (Scopus)

Abstract

Recently, the incidence of reported pertussis cases of adults has dramatically increased in Japan. In the present study, we analyzed seven Bordetella pertussis isolates recovered from adults in Japan using pulsed-field gel electrophoresis (PFGE) and sequencing of their antigenic and virulence-associated proteins, compared with those from children. PFGE analysis demonstrated that the adult strains were closely related to the child strains (78-100% genetic similarity). On the other hand, the genotyping revealed that 71% (5/7) of the adult strains and 47% (25/53) of the child strains had the same combination of antigenic/virulence-associated allelic variants (ptxS1B/prn1/fim2-1/fim3A/fhaB1/tcfA2) as the Japanese vaccine strain Tohama, respectively. In comparison to the child strains, there was no apparent antigenic and genetic shift in the adult strains. Our result suggests that (i) there is no B. pertussis circulating strain specific to adults and (ii) the antigenic/virulence-associated proteins are unrelated to the rise in adult pertussis incidence in Japan.

Original languageEnglish
Pages (from-to)1530-1534
Number of pages5
JournalVaccine
Volume26
Issue number12
DOIs
Publication statusPublished - 17-03-2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • General Immunology and Microbiology
  • General Veterinary
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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