Antitumor effect of intra-arterial tumor necrosis factor-α in rats with transplanted intracerebral glioma and its evaluation by MRI

K. Harada, J. Yoshida, T. Wakabayashi, K. Sugita, K. Kurisu, T. Uozumi, B. F. Zieroth, M. Takahashi, T. Yamanaka

Research output: Contribution to journalArticle

Abstract

Recombinant human TNF-α was administered intraarterially to rats with transplanted intracerebral glioma, 1 x 106 of T9 rat glioma cell were transplanted into Fisher 344 rat brain stereotaxically and 1000units of TNF- α was administered at a rate of 100 μl/min, via an internal carotid artery 1 or 3 weeks after the transplantation. The effects of TNF-α were evaluated by MRI and histopathological examinations. Neurological symptoms, i.e., hemiparesis, appeared after 9.0±0.63 days and all rats died of tumor overloading 14.5±0.84 days after the transplantation. Single injection of TNF-α on 7th day after the transplantation induced regression of the tumor size in one of six rats. The tumors were detected 3 days after transplantation by MRI and they were revealed as low/iso intensity mass in T1W1, iso/high intensity in T2W1, and were enhanced by Gd-DTPA heterogeneously. On 7/14 days after the transplantation, the tumor grew approximately 7/10mm in diameter. The single 1000 units of TNF-α were administered via an internal carotid artery. 3 days after the administration of TNF-α, regression of the tumor size was seen in one of six rats and decrease of peritumoral edema was seen in three. These effects of TNF-α were, however, transient and they were not demonstrated on day 7. Single injection of TNF-α was not effective for large tumors more than 10mm in diameter seen 14 days after the transplantation. These data suggest that intra-arterial TNF-α should be administered at an early stage of the tumor growth and several injections are needed to cause regression in the size of the gliomas.

Original languageEnglish
Pages (from-to)1069-1074
Number of pages6
JournalNeurological Surgery
Volume23
Issue number12
Publication statusPublished - 01-01-1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Clinical Neurology

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    Harada, K., Yoshida, J., Wakabayashi, T., Sugita, K., Kurisu, K., Uozumi, T., Zieroth, B. F., Takahashi, M., & Yamanaka, T. (1995). Antitumor effect of intra-arterial tumor necrosis factor-α in rats with transplanted intracerebral glioma and its evaluation by MRI. Neurological Surgery, 23(12), 1069-1074.