Apoptotic signaling pathway activated by Helicobacter pylori infection and increase of apoptosis-inducing activity under serum-starved conditions

K. Shibayama, Yohei Doi, N. Shibata, T. Yagi, T. Nada, Y. Iinuma, Y. Arakawa

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The enhanced gastric epithelial cell apoptosis observed during infection with Helicobacter pylori has been suggested to be of significance in the etiology of gastritis, peptic ulcers, and neoplasia. To investigate the cell death signaling induced by H. pylori infection, human gastric epithelial cells were incubated with H. pylori for up to 72 h. H. pylori infection induced the activation of caspase -8, -9, and -3 and the expression of the proapoptotic Bcl-2 family proteins Bad and Bid. The peak of the activity of the caspases occurred at 24 h. At this time, the inhibition of caspase-8 or -9 almost completely suppressed H. pylori-induced apoptosis. Inhibition of caspase-8 suppressed the expression of Bad and Bid and the subsequent activation of caspase-9 and -3. These observations indicate that H. pylori induces apoptosis through a pathway involving the sequential induction of apical caspase-8 activity, the proapoptotic proteins Bad and Bid, caspase-9 activity, and effector caspase-3 activity. Activation of the pathway was independent of CagA or vacuolating toxin. A membrane fraction of H. pylori was sufficient to activate this pathway, and treatment with proteinase K eliminated the activity. Apoptotic activity of the membrane fraction was significantly increased by incubating the bacteria under serum-starved conditions for 24 h. These observations suggest that environmental conditions in the human stomach could induce H. pylori-mediated pathogenesis, leading to a variety of clinical outcomes.

Original languageEnglish
Pages (from-to)3181-3189
Number of pages9
JournalInfection and Immunity
Volume69
Issue number5
DOIs
Publication statusPublished - 02-05-2001

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Helicobacter Infections
Helicobacter pylori
Apoptosis
Caspase 8
Serum
Caspase 9
BH3 Interacting Domain Death Agonist Protein
bcl-Associated Death Protein
Stomach
Caspase 3
Epithelial Cells
Effector Caspases
Endopeptidase K
Membranes
Gastritis
Caspases
Peptic Ulcer
Cell Death
Bacteria
Infection

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Shibayama, K. ; Doi, Yohei ; Shibata, N. ; Yagi, T. ; Nada, T. ; Iinuma, Y. ; Arakawa, Y. / Apoptotic signaling pathway activated by Helicobacter pylori infection and increase of apoptosis-inducing activity under serum-starved conditions. In: Infection and Immunity. 2001 ; Vol. 69, No. 5. pp. 3181-3189.
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Apoptotic signaling pathway activated by Helicobacter pylori infection and increase of apoptosis-inducing activity under serum-starved conditions. / Shibayama, K.; Doi, Yohei; Shibata, N.; Yagi, T.; Nada, T.; Iinuma, Y.; Arakawa, Y.

In: Infection and Immunity, Vol. 69, No. 5, 02.05.2001, p. 3181-3189.

Research output: Contribution to journalArticle

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AU - Shibayama, K.

AU - Doi, Yohei

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