Application of efficient and specific gene transfer systems and organ culture techniques for the elucidation of mechanisms of epithelial-mesenchymal interaction in the developing gut

Kimiko Fukuda, Nobuyuki Sakamoto, Tomohiro Narita, Kanako Saitoh, Takashi Kameda, Hideo Iba, Sadao Yasugi

Research output: Contribution to journalShort surveypeer-review

35 Citations (Scopus)

Abstract

Epithelial-mesenchymal interactions are very important in the development of the vertebrate gut. In the avian embryonic stomach (proventriculus), expression of embryonic chick pepsinogen (ECPg) gene, which is specific to developing glandular cells in stomach epithelium, is regulated by mesenchymal influence. Molecular mechanisms of tissue-specific transcriptional regulation of the ECPg gene and the molecular nature of the mesenchymal signals were analyzed using a combination of the classic organ culture system and gene transfer strategies. In the present review, three methods for the introduction of DNA into tissues are described: lipofection, electroporation and retroviral infection, and characteristics of each system are discussed.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalDevelopment Growth and Differentiation
Volume42
Issue number3
DOIs
Publication statusPublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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