Appropriate nonwoven filters effectively capture human peripheral blood cells and mesenchymal stem cells, which show enhanced production of growth factors

Hideo Hori, Ushio Iwamoto, Gen Niimi, Masanori Shinzato, Yoshiyuki Hiki, Yasuo Tokushima, Kazunori Kawaguchi, Atsushi Ohashi, Shigeru Nakai, Mikitomo Yasutake, Nobuya Kitaguchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2–22 μm fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2–1.8 μm captured over 70 % of leukocytes or 90 % of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor β1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalJournal of Artificial Organs
Volume18
Issue number1
DOIs
Publication statusPublished - 01-01-2015

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Polyethylene Terephthalates
Blood Cells
Intercellular Signaling Peptides and Proteins
Blood
Cells
Fibers
Tissue
Polyethylene terephthalates
Scaffolds
Vascular Endothelial Growth Factor A
Acids
Chitin
Fibroblasts
Conditioned Culture Medium
Equipment and Supplies
Regenerative Medicine
Transforming Growth Factors
poly(lactic acid)

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine

Cite this

Hori, Hideo ; Iwamoto, Ushio ; Niimi, Gen ; Shinzato, Masanori ; Hiki, Yoshiyuki ; Tokushima, Yasuo ; Kawaguchi, Kazunori ; Ohashi, Atsushi ; Nakai, Shigeru ; Yasutake, Mikitomo ; Kitaguchi, Nobuya. / Appropriate nonwoven filters effectively capture human peripheral blood cells and mesenchymal stem cells, which show enhanced production of growth factors. In: Journal of Artificial Organs. 2015 ; Vol. 18, No. 1. pp. 55-63.
@article{b6e7d7402b7e485baf16486b6e133119,
title = "Appropriate nonwoven filters effectively capture human peripheral blood cells and mesenchymal stem cells, which show enhanced production of growth factors",
abstract = "Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2–22 μm fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2–1.8 μm captured over 70 {\%} of leukocytes or 90 {\%} of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor β1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.",
author = "Hideo Hori and Ushio Iwamoto and Gen Niimi and Masanori Shinzato and Yoshiyuki Hiki and Yasuo Tokushima and Kazunori Kawaguchi and Atsushi Ohashi and Shigeru Nakai and Mikitomo Yasutake and Nobuya Kitaguchi",
year = "2015",
month = "1",
day = "1",
doi = "10.1007/s10047-014-0794-9",
language = "English",
volume = "18",
pages = "55--63",
journal = "Journal of Artificial Organs",
issn = "1434-7229",
publisher = "Springer Japan",
number = "1",

}

Appropriate nonwoven filters effectively capture human peripheral blood cells and mesenchymal stem cells, which show enhanced production of growth factors. / Hori, Hideo; Iwamoto, Ushio; Niimi, Gen; Shinzato, Masanori; Hiki, Yoshiyuki; Tokushima, Yasuo; Kawaguchi, Kazunori; Ohashi, Atsushi; Nakai, Shigeru; Yasutake, Mikitomo; Kitaguchi, Nobuya.

In: Journal of Artificial Organs, Vol. 18, No. 1, 01.01.2015, p. 55-63.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Appropriate nonwoven filters effectively capture human peripheral blood cells and mesenchymal stem cells, which show enhanced production of growth factors

AU - Hori, Hideo

AU - Iwamoto, Ushio

AU - Niimi, Gen

AU - Shinzato, Masanori

AU - Hiki, Yoshiyuki

AU - Tokushima, Yasuo

AU - Kawaguchi, Kazunori

AU - Ohashi, Atsushi

AU - Nakai, Shigeru

AU - Yasutake, Mikitomo

AU - Kitaguchi, Nobuya

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2–22 μm fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2–1.8 μm captured over 70 % of leukocytes or 90 % of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor β1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.

AB - Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2–22 μm fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2–1.8 μm captured over 70 % of leukocytes or 90 % of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor β1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.

UR - http://www.scopus.com/inward/record.url?scp=84924112712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924112712&partnerID=8YFLogxK

U2 - 10.1007/s10047-014-0794-9

DO - 10.1007/s10047-014-0794-9

M3 - Article

C2 - 25322703

AN - SCOPUS:84924112712

VL - 18

SP - 55

EP - 63

JO - Journal of Artificial Organs

JF - Journal of Artificial Organs

SN - 1434-7229

IS - 1

ER -