Aqueous fraction of Sauropus androgynus might be responsible for bronchiolitis obliterans

Izumi Hashimoto, Kazuyoshi Imaizumi, Naozumi Hashimoto, Hiroshi Furukawa, Yukihiro Noda, Tsutomu Kawabe, Toyohiro Honda, Tomomi Ogawa, Masaki Matsuo, Naoyuki Imai, Satoru Ito, Mitsuo Sato, Masashi Kondo, Kaoru Shimokata, Yoshinori Hasegawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background and objective: Bronchiolitis obliterans (BO) has been reported to develop following ingestion of Sauropus androgynus (SA), a leafy shrub distributed in Southeast Asia. Little is known about direct effects of SA on airway resident cells or haematopoietic cells in vitro. Identification of the SA component responsible for the development of BO would be an important key to elucidate its mechanism. We sought to elucidate the direct effects of SA on airway resident cells or haematopoietic cells and identify the SA element responsible for the pathogenesis of BO. Methods: SA dry powder was partitioned into fractions by solvent extraction. Human and murine monocytic cells, epithelial cells and endothelial cells were cultured with SA solution or fractions eluted from SA. We also investigated the effect of SA in vivo using a murine BO syndrome (BOS) model. Results: The aqueous fraction of SA induced significant increases of inflammatory cytokine and chemokine production from monocytic lineage cells. This fraction also induced significant apoptosis of endothelial cells and enhanced intraluminal obstructive fibrosis in allogeneic trachea allograft in the murine BOS model. We found individual differences in tumour necrosis factor α (TNF-α) production from monocytes of healthy controls stimulated by this aqueous fraction of SA, whereas it induced high-level TNF-α production from monocytes of patients with SA-induced BO. Conclusions: These results suggest that an aqueous fraction of SA may be responsible for the pathogenesis of BO. Ingestion of Sauropus androgynus (SA) as dry powder or fresh juice has been reported to induce constrictive bronchiolitis obliterans (BO), but the pathogenesis of this condition is unknown. This study suggests that an aqueous fraction of SA could be responsible for development of SA-induced BO.

Original languageEnglish
Pages (from-to)340-347
Number of pages8
JournalRespirology
Volume18
Issue number2
DOIs
Publication statusPublished - 01-02-2013

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Bronchiolitis Obliterans
Powders
Monocytes
Endothelial Cells
Tumor Necrosis Factor-alpha
Eating
Southeastern Asia
Trachea
Chemokines
Individuality
Allografts
Fibrosis
Epithelial Cells
Apoptosis
Cytokines

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Hashimoto, I., Imaizumi, K., Hashimoto, N., Furukawa, H., Noda, Y., Kawabe, T., ... Hasegawa, Y. (2013). Aqueous fraction of Sauropus androgynus might be responsible for bronchiolitis obliterans. Respirology, 18(2), 340-347. https://doi.org/10.1111/j.1440-1843.2012.02286.x
Hashimoto, Izumi ; Imaizumi, Kazuyoshi ; Hashimoto, Naozumi ; Furukawa, Hiroshi ; Noda, Yukihiro ; Kawabe, Tsutomu ; Honda, Toyohiro ; Ogawa, Tomomi ; Matsuo, Masaki ; Imai, Naoyuki ; Ito, Satoru ; Sato, Mitsuo ; Kondo, Masashi ; Shimokata, Kaoru ; Hasegawa, Yoshinori. / Aqueous fraction of Sauropus androgynus might be responsible for bronchiolitis obliterans. In: Respirology. 2013 ; Vol. 18, No. 2. pp. 340-347.
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abstract = "Background and objective: Bronchiolitis obliterans (BO) has been reported to develop following ingestion of Sauropus androgynus (SA), a leafy shrub distributed in Southeast Asia. Little is known about direct effects of SA on airway resident cells or haematopoietic cells in vitro. Identification of the SA component responsible for the development of BO would be an important key to elucidate its mechanism. We sought to elucidate the direct effects of SA on airway resident cells or haematopoietic cells and identify the SA element responsible for the pathogenesis of BO. Methods: SA dry powder was partitioned into fractions by solvent extraction. Human and murine monocytic cells, epithelial cells and endothelial cells were cultured with SA solution or fractions eluted from SA. We also investigated the effect of SA in vivo using a murine BO syndrome (BOS) model. Results: The aqueous fraction of SA induced significant increases of inflammatory cytokine and chemokine production from monocytic lineage cells. This fraction also induced significant apoptosis of endothelial cells and enhanced intraluminal obstructive fibrosis in allogeneic trachea allograft in the murine BOS model. We found individual differences in tumour necrosis factor α (TNF-α) production from monocytes of healthy controls stimulated by this aqueous fraction of SA, whereas it induced high-level TNF-α production from monocytes of patients with SA-induced BO. Conclusions: These results suggest that an aqueous fraction of SA may be responsible for the pathogenesis of BO. Ingestion of Sauropus androgynus (SA) as dry powder or fresh juice has been reported to induce constrictive bronchiolitis obliterans (BO), but the pathogenesis of this condition is unknown. This study suggests that an aqueous fraction of SA could be responsible for development of SA-induced BO.",
author = "Izumi Hashimoto and Kazuyoshi Imaizumi and Naozumi Hashimoto and Hiroshi Furukawa and Yukihiro Noda and Tsutomu Kawabe and Toyohiro Honda and Tomomi Ogawa and Masaki Matsuo and Naoyuki Imai and Satoru Ito and Mitsuo Sato and Masashi Kondo and Kaoru Shimokata and Yoshinori Hasegawa",
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Hashimoto, I, Imaizumi, K, Hashimoto, N, Furukawa, H, Noda, Y, Kawabe, T, Honda, T, Ogawa, T, Matsuo, M, Imai, N, Ito, S, Sato, M, Kondo, M, Shimokata, K & Hasegawa, Y 2013, 'Aqueous fraction of Sauropus androgynus might be responsible for bronchiolitis obliterans', Respirology, vol. 18, no. 2, pp. 340-347. https://doi.org/10.1111/j.1440-1843.2012.02286.x

Aqueous fraction of Sauropus androgynus might be responsible for bronchiolitis obliterans. / Hashimoto, Izumi; Imaizumi, Kazuyoshi; Hashimoto, Naozumi; Furukawa, Hiroshi; Noda, Yukihiro; Kawabe, Tsutomu; Honda, Toyohiro; Ogawa, Tomomi; Matsuo, Masaki; Imai, Naoyuki; Ito, Satoru; Sato, Mitsuo; Kondo, Masashi; Shimokata, Kaoru; Hasegawa, Yoshinori.

In: Respirology, Vol. 18, No. 2, 01.02.2013, p. 340-347.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Aqueous fraction of Sauropus androgynus might be responsible for bronchiolitis obliterans

AU - Hashimoto, Izumi

AU - Imaizumi, Kazuyoshi

AU - Hashimoto, Naozumi

AU - Furukawa, Hiroshi

AU - Noda, Yukihiro

AU - Kawabe, Tsutomu

AU - Honda, Toyohiro

AU - Ogawa, Tomomi

AU - Matsuo, Masaki

AU - Imai, Naoyuki

AU - Ito, Satoru

AU - Sato, Mitsuo

AU - Kondo, Masashi

AU - Shimokata, Kaoru

AU - Hasegawa, Yoshinori

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Background and objective: Bronchiolitis obliterans (BO) has been reported to develop following ingestion of Sauropus androgynus (SA), a leafy shrub distributed in Southeast Asia. Little is known about direct effects of SA on airway resident cells or haematopoietic cells in vitro. Identification of the SA component responsible for the development of BO would be an important key to elucidate its mechanism. We sought to elucidate the direct effects of SA on airway resident cells or haematopoietic cells and identify the SA element responsible for the pathogenesis of BO. Methods: SA dry powder was partitioned into fractions by solvent extraction. Human and murine monocytic cells, epithelial cells and endothelial cells were cultured with SA solution or fractions eluted from SA. We also investigated the effect of SA in vivo using a murine BO syndrome (BOS) model. Results: The aqueous fraction of SA induced significant increases of inflammatory cytokine and chemokine production from monocytic lineage cells. This fraction also induced significant apoptosis of endothelial cells and enhanced intraluminal obstructive fibrosis in allogeneic trachea allograft in the murine BOS model. We found individual differences in tumour necrosis factor α (TNF-α) production from monocytes of healthy controls stimulated by this aqueous fraction of SA, whereas it induced high-level TNF-α production from monocytes of patients with SA-induced BO. Conclusions: These results suggest that an aqueous fraction of SA may be responsible for the pathogenesis of BO. Ingestion of Sauropus androgynus (SA) as dry powder or fresh juice has been reported to induce constrictive bronchiolitis obliterans (BO), but the pathogenesis of this condition is unknown. This study suggests that an aqueous fraction of SA could be responsible for development of SA-induced BO.

AB - Background and objective: Bronchiolitis obliterans (BO) has been reported to develop following ingestion of Sauropus androgynus (SA), a leafy shrub distributed in Southeast Asia. Little is known about direct effects of SA on airway resident cells or haematopoietic cells in vitro. Identification of the SA component responsible for the development of BO would be an important key to elucidate its mechanism. We sought to elucidate the direct effects of SA on airway resident cells or haematopoietic cells and identify the SA element responsible for the pathogenesis of BO. Methods: SA dry powder was partitioned into fractions by solvent extraction. Human and murine monocytic cells, epithelial cells and endothelial cells were cultured with SA solution or fractions eluted from SA. We also investigated the effect of SA in vivo using a murine BO syndrome (BOS) model. Results: The aqueous fraction of SA induced significant increases of inflammatory cytokine and chemokine production from monocytic lineage cells. This fraction also induced significant apoptosis of endothelial cells and enhanced intraluminal obstructive fibrosis in allogeneic trachea allograft in the murine BOS model. We found individual differences in tumour necrosis factor α (TNF-α) production from monocytes of healthy controls stimulated by this aqueous fraction of SA, whereas it induced high-level TNF-α production from monocytes of patients with SA-induced BO. Conclusions: These results suggest that an aqueous fraction of SA may be responsible for the pathogenesis of BO. Ingestion of Sauropus androgynus (SA) as dry powder or fresh juice has been reported to induce constrictive bronchiolitis obliterans (BO), but the pathogenesis of this condition is unknown. This study suggests that an aqueous fraction of SA could be responsible for development of SA-induced BO.

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Hashimoto I, Imaizumi K, Hashimoto N, Furukawa H, Noda Y, Kawabe T et al. Aqueous fraction of Sauropus androgynus might be responsible for bronchiolitis obliterans. Respirology. 2013 Feb 1;18(2):340-347. https://doi.org/10.1111/j.1440-1843.2012.02286.x