Arginine methylation of ubiquitin-associated protein 2-like is required for the accurate distribution of chromosomes

Masao Maeda, Hitoki Hasegawa, Mai Sugiyama, Toshinori Hyodo, Satoko Ito, Dan Chen, Eri Asano, Akio Masuda, Yoshinori Hasegawa, Michinari Hamaguchi, Takeshi Senga

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Proper bioriented attachment of microtubules and kinetochores is essential for the precise distribution of duplicated chromosomes to each daughter cell. An aberrant kinetochore-microtubule attachment results in chromosome instability, which leads to cellular transformation or apoptosis. In this article, we show that ubiquitin-associated protein 2-like (UBAP2L) is necessary for correct kinetochore-microtubule attachment. Depletion of UBAP2L inhibited chromosome alignment in metaphase and delayed progression to anaphase by activating spindle assembly checkpoint signaling. In addition, UBAP2L knockdown increased side-on attachment of kinetochores along the microtubules and suppressed stable kinetochore fiber formation. A proteomics analysis identified protein arginine methyltransferase (PRMT)1 as a direct interaction partner of UBAP2L. UBAP2L has an arginine- and glycine-rich motif called the RGG/RG or GAR motif in the N terminus. Biochemical analysis confirmed that arginine residues in the RGG/RG motif of UBAP2L were directly methylated by PRMT1. Finally, we demonstrated that the RGG/RG motif of UBAP2L is essential for the proper alignment of chromosomes in metaphase for the accurate distribution of chromosomes. Our results show a possible role for arginine methylation in UBAP2L for the progression of mitosis.

Original languageEnglish
Pages (from-to)312-323
Number of pages12
JournalFASEB Journal
Volume30
Issue number1
DOIs
Publication statusPublished - 01-01-2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Arginine methylation of ubiquitin-associated protein 2-like is required for the accurate distribution of chromosomes'. Together they form a unique fingerprint.

Cite this