Arsenic trioxide induces apoptosis in neuroblastoma cell lines through the activation of caspase 3 in vitro

Yukihiro Akao, Yoshihito Nakagawa, Kiyotaka Akiyama

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

Arsenic trioxide (As2O3) induces clinical remission in acute promyelocytic leukemia, even in all-trans retinoic acid-refractory cases, with minimal toxicity at low (1-2 μM) concentration. We exposed various neuroblastoma cell lines to As2O3 at a concentration of 2 μM: as a result, seven of 10 neuroblastoma cell lines underwent apoptosis characterized by morphological changes and nucleosomal DNA fragmentation. As2O3-induced apoptosis in neuroblastoma cells was shown to occur through the activation of caspase 3, as judged from Western blot analysis and apoptosis inhibition assay. It seemed that the sensitivity of neuroblastoma cells to As2O3 was inversely proportional to their intracellular level of reduced glutathione. Taken together these results indicate that As2O3 would be a candidate as a therapeutic agent for treatment of neuroblastoma, which is a solid tumor, not only by systemic therapy but also by local therapy. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalFEBS Letters
Volume455
Issue number1-2
DOIs
Publication statusPublished - 16-07-1999

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Neuroblastoma
Caspase 3
Chemical activation
Cells
Apoptosis
Cell Line
Acute Promyelocytic Leukemia
DNA Fragmentation
Tretinoin
Refractory materials
Glutathione
Toxicity
In Vitro Techniques
arsenic trioxide
Tumors
Assays
Therapeutics
Western Blotting
DNA
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

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abstract = "Arsenic trioxide (As2O3) induces clinical remission in acute promyelocytic leukemia, even in all-trans retinoic acid-refractory cases, with minimal toxicity at low (1-2 μM) concentration. We exposed various neuroblastoma cell lines to As2O3 at a concentration of 2 μM: as a result, seven of 10 neuroblastoma cell lines underwent apoptosis characterized by morphological changes and nucleosomal DNA fragmentation. As2O3-induced apoptosis in neuroblastoma cells was shown to occur through the activation of caspase 3, as judged from Western blot analysis and apoptosis inhibition assay. It seemed that the sensitivity of neuroblastoma cells to As2O3 was inversely proportional to their intracellular level of reduced glutathione. Taken together these results indicate that As2O3 would be a candidate as a therapeutic agent for treatment of neuroblastoma, which is a solid tumor, not only by systemic therapy but also by local therapy. Copyright (C) 1999 Federation of European Biochemical Societies.",
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Arsenic trioxide induces apoptosis in neuroblastoma cell lines through the activation of caspase 3 in vitro. / Akao, Yukihiro; Nakagawa, Yoshihito; Akiyama, Kiyotaka.

In: FEBS Letters, Vol. 455, No. 1-2, 16.07.1999, p. 59-62.

Research output: Contribution to journalArticle

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