Ascorbic acid improves postischemic vasodilatation impaired by infusion of soybean oil into canine iliac artery

Hiroyuki Osanai, Kenji Okumura, Makoto Hayakawa, Mitsunori Harada, Yasushi Numaguchi, Shinji Mokuno, Kichiro Murase, Hideo Matsui, Yukio Toki, Takayuki Ito, Tetsuo Hayakawa

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10 Citations (Scopus)

Abstract

This study was conducted to (a) assess postischemic vasodilatation by changes in the vascular cross-sectional area using simultaneous intravascular two-dimensional and Doppler ultrasound before and after the infusion of Intralipid (Pharmacia and Upjohn, Peapack, NJ, U.S.A.); (b) evaluate how antioxidant ascorbic acid modifies the effects of Intralipid on postischemic vasodilatation; and (c) clarify the changes in plasma nitrite and nitrate (NOx-) levels after the infusion of Intralipid with and without ascorbic acid. Twenty-eight mongrel dogs were used to measure for vascular cross-sectional area and average instantaneous peak velocity in the iliac arteries after the 5-min occlusion of the arteries. Postischemic vasodilatation was impaired after the infusion of Intralipid (20%, 2 ml/kg) and this impaired response was reversed by the co-administration of ascorbic acid (30 mg/kg). N(G)-monomethyl-L-arginine completely abolished postischemic vasodilatation. Plasma NOx- levels were significantly reduced after the infusion of Intralipid compared with baseline (11.6 ± 0.4 vs. 12.9 ± 0.3 μM, p = 0.025) and after infusion of Intralipid with ascorbic acid compared with baseline (11.8 ± 0.5 vs. 13.1 ± 0.4 μM, p = 0.047). We concluded that ascorbic acid reverses the endothelial dysfunction induced by Intralipid without increasing plasma NOx- levels and that deactivation of nitric oxide by oxidative stress is a primary contributor to Intralipid-induced impaired vasodilation.

Original languageEnglish
Pages (from-to)687-692
Number of pages6
JournalJournal of Cardiovascular Pharmacology
Volume36
Issue number6
DOIs
Publication statusPublished - 01-01-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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    Osanai, H., Okumura, K., Hayakawa, M., Harada, M., Numaguchi, Y., Mokuno, S., Murase, K., Matsui, H., Toki, Y., Ito, T., & Hayakawa, T. (2000). Ascorbic acid improves postischemic vasodilatation impaired by infusion of soybean oil into canine iliac artery. Journal of Cardiovascular Pharmacology, 36(6), 687-692. https://doi.org/10.1097/00005344-200012000-00001