Assembly of 100 nm periodic fibrils (type VI collagen) in human infant corneal stroma

M. Nakamura, M. Kobayashi, K. Hirano, K. Kobayashi, T. Hoshino, S. Awaya

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8 Citations (Scopus)


An experimental model for the age-related changes in the extracellular matrix of the human cornea was developed. Human infant corneal stroma in which no long-spacing collagen occurred naturally was treated with 20 mM adenosine triphosphate (ATP). The ATP-treated cornea was observed and compared with nontreated control specimens by electron microscopy. Numerous 100 nm periodic fibrils which resembled long-spacing collagen were formed by the treatment. These experimentally formed fibrils appeared to attract and aggregate the collagen fibrils with D-periodicity, which indicated a connection between type VI and type I collagen. By ruthenium red staining, the cross-bands of the 100 nm periodic fibrils were positively stained, indicating that glycosaminoglycans or proteoglycans were involved in the formation of these fibrils.

Original languageEnglish
Pages (from-to)458-464
Number of pages7
JournalJapanese Journal of Ophthalmology
Issue number4
Publication statusPublished - 01-12-1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Ophthalmology


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