Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

Vincent VanBuren; Yulan Piao; Dawood B. Dudekula; Yong Qian; Carter G. Carter; Patrick R. Martin; Carole A. Stagg; Uwem C. Bassey; Kazuhiro Aiba; Toshio Hamatani; George J. Kargul; Amber G. Luo; Janet Kelso; Winston Hide; Minoru S.H. Ko

doi:10.1101/gr.633802

Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

Vincent VanBuren, Yulan Piao, Dawood B. Dudekula, Yong Qian, Carter G. Carter, Patrick R. Martin, Carole A. Stagg, Uwem C. Bassey, Kazuhiro Aiba, Toshio Hamatani, George J. Kargul, Amber G. Luo, Janet Kelso, Winston Hide, Minoru S.H. Ko

Research output: Contribution to journal › Review article › peer-review

44 Citations (Scopus)

Abstract

A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

Original language	English
Pages (from-to)	1999-2003
Number of pages	5
Journal	Genome Research
Volume	12
Issue number	12
DOIs	https://doi.org/10.1101/gr.633802
Publication status	Published - 01-12-2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics
Genetics(clinical)

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10.1101/gr.633802

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title = "Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set",

abstract = "A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.",

author = "Vincent VanBuren and Yulan Piao and Dudekula, {Dawood B.} and Yong Qian and Carter, {Carter G.} and Martin, {Patrick R.} and Stagg, {Carole A.} and Bassey, {Uwem C.} and Kazuhiro Aiba and Toshio Hamatani and Kargul, {George J.} and Luo, {Amber G.} and Janet Kelso and Winston Hide and Ko, {Minoru S.H.}",

year = "2002",

month = dec,

day = "1",

doi = "10.1101/gr.633802",

language = "English",

volume = "12",

pages = "1999--2003",

journal = "Genome Research",

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T1 - Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

AU - VanBuren, Vincent

AU - Piao, Yulan

AU - Dudekula, Dawood B.

AU - Qian, Yong

AU - Carter, Carter G.

AU - Martin, Patrick R.

AU - Stagg, Carole A.

AU - Bassey, Uwem C.

AU - Aiba, Kazuhiro

AU - Hamatani, Toshio

AU - Kargul, George J.

AU - Luo, Amber G.

AU - Kelso, Janet

AU - Hide, Winston

AU - Ko, Minoru S.H.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

AB - A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

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DO - 10.1101/gr.633802

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SN - 1088-9051

VL - 12

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EP - 2003

JO - Genome Research

JF - Genome Research

IS - 12

ER -

Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

Abstract

All Science Journal Classification (ASJC) codes

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