Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

Vincent VanBuren; Yulan Piao; Dawood B. Dudekula; Yong Qian; Carter G. Carter; Patrick R. Martin; Carole A. Stagg; Uwem C. Bassey; Kazuhiro Aiba; Toshio Hamatani; George J. Kargul; Amber G. Luo; Janet Kelso; Winston Hide; Minoru S.H. Ko

doi:10.1101/gr.633802

Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

Vincent VanBuren
, Yulan Piao
, Dawood B. Dudekula
, Yong Qian
, Carter G. Carter
, Patrick R. Martin
, Carole A. Stagg
, Uwem C. Bassey
, Kazuhiro Aiba
, Toshio Hamatani
, George J. Kargul
, Amber G. Luo
, Janet Kelso
, Winston Hide
, Minoru S.H. Ko

Research output: Contribution to journal › Review article › peer-review

45 Citations (Scopus)

Abstract

A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

Original language	English
Pages (from-to)	1999-2003
Number of pages	5
Journal	Genome Research
Volume	12
Issue number	12
DOIs	https://doi.org/10.1101/gr.633802
Publication status	Published - 01-12-2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics
Genetics(clinical)

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10.1101/gr.633802

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@article{c5089943201f4272a0dffd12c8227bc6,

title = "Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set",

abstract = "A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.",

author = "Vincent VanBuren and Yulan Piao and Dudekula, \{Dawood B.\} and Yong Qian and Carter, \{Carter G.\} and Martin, \{Patrick R.\} and Stagg, \{Carole A.\} and Bassey, \{Uwem C.\} and Kazuhiro Aiba and Toshio Hamatani and Kargul, \{George J.\} and Luo, \{Amber G.\} and Janet Kelso and Winston Hide and Ko, \{Minoru S.H.\}",

year = "2002",

month = dec,

day = "1",

doi = "10.1101/gr.633802",

language = "English",

volume = "12",

pages = "1999--2003",

journal = "Genome Research",

issn = "1088-9051",

publisher = "Cold Spring Harbor Laboratory Press",

number = "12",

}

TY - JOUR

T1 - Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

AU - VanBuren, Vincent

AU - Piao, Yulan

AU - Dudekula, Dawood B.

AU - Qian, Yong

AU - Carter, Carter G.

AU - Martin, Patrick R.

AU - Stagg, Carole A.

AU - Bassey, Uwem C.

AU - Aiba, Kazuhiro

AU - Hamatani, Toshio

AU - Kargul, George J.

AU - Luo, Amber G.

AU - Kelso, Janet

AU - Hide, Winston

AU - Ko, Minoru S.H.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

AB - A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

UR - https://www.scopus.com/pages/publications/0036907106

UR - https://www.scopus.com/pages/publications/0036907106#tab=citedBy

U2 - 10.1101/gr.633802

DO - 10.1101/gr.633802

M3 - Review article

C2 - 12466305

AN - SCOPUS:0036907106

SN - 1088-9051

VL - 12

SP - 1999

EP - 2003

JO - Genome Research

JF - Genome Research

IS - 12

ER -

Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

Abstract

All Science Journal Classification (ASJC) codes

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