Association between common genetic variants in pre-microRNAs and the clinicopathological characteristics and survival of gastric cancer patients

Masaaki Okubo, Tomomitsu Tahara, Tomoyuki Shibata, Hiromi Yamashita, Masakatsu Nakamura, Daisuke Yoshioka, Joh Yonemura, Yoshio Kamiya, Takamitsu Ishizuka, Yoshihito Nakagawa, Mitsuo Nagasaka, Masami Iwata, Tomiyasu Arisawa, Ichiro Hirata

Research output: Contribution to journalArticle

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Abstract

Common single-nucleotide polymorphisms (SNPs) in micrornas (miRNAs) have been shown to be associated with susceptibility to several types of human cancer. We evaluated the association between three SNPs (rs11614913, rs2910164 and rs3746444) in pre-miRNAs (mir-196a2, mir-146a and mir-499) and various clinicopathological characteristics, including CpG island hypermethylation (CIHM) status and overall survival in gastric cancer (GC) patients. rs11614913 (T>C), rs2910164 (C>G) and rs3746444 (A>G) SNPs were genotyped in 127 Gc patients. cihm of p14, p16, dap-kinase and cdh1 genes was determined by methylation-specific polymerase chain reaction in the cancer tissues. A significant marginal association was found between the rs11614913 CC genotype and polypoid or elevated type morphology in early-stage GC (Or=6.29, 95% CI 1.18-33.47, p=0.03). the rs2910164 CC and CG genotypes were associated with increased susceptibility to cihm of dap-kinase (CC+CG, OR=5.48, 95% CI 1.30-23.10, p=0.02; CC, OR=6.93, 95% CI 1.37-35.02, P=0.02; CG, OR=4.24, 95% CI 0.87-20.78, p=0.07). the 11614913 TT and TC genotypes were associated with a higher number of CIHM (no. of CIHM 0-1 vs. 2-4; TT+TC, OR=3.67, 95% CI 0.98-13.72, p=0.05; TC, OR=4.08, 95% CI 1.04-15.97, p=0.04). When the subjects were divided according to age group, the combined rs11614913 TT+TC genotype tended to be associated with worse overall survival than the CC genotype in patients younger than 65 years of age (p=0.05). The combined rs2910164 CG+GG genotype also tended to be associated with worse overall survival than the CC genotype in the same age group (p=0.09). It appears that rs11614913 and rs2910164 SNPNPs in pre-miRNAs (miR-196a2 and miR-146a) affect the clinicopathological characteristics of GC, including its morphological appearance, CIHM status and overall survival.

Original languageEnglish
Pages (from-to)1035-1040
Number of pages6
JournalExperimental and Therapeutic Medicine
Volume1
Issue number6
DOIs
Publication statusPublished - 01-11-2010

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MicroRNAs
Stomach Neoplasms
Genotype
CpG Islands
Survival
Single Nucleotide Polymorphism
Phosphotransferases
Age Groups
Methylation
Neoplasms
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology (miscellaneous)
  • Cancer Research

Cite this

Okubo, Masaaki ; Tahara, Tomomitsu ; Shibata, Tomoyuki ; Yamashita, Hiromi ; Nakamura, Masakatsu ; Yoshioka, Daisuke ; Yonemura, Joh ; Kamiya, Yoshio ; Ishizuka, Takamitsu ; Nakagawa, Yoshihito ; Nagasaka, Mitsuo ; Iwata, Masami ; Arisawa, Tomiyasu ; Hirata, Ichiro. / Association between common genetic variants in pre-microRNAs and the clinicopathological characteristics and survival of gastric cancer patients. In: Experimental and Therapeutic Medicine. 2010 ; Vol. 1, No. 6. pp. 1035-1040.
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author = "Masaaki Okubo and Tomomitsu Tahara and Tomoyuki Shibata and Hiromi Yamashita and Masakatsu Nakamura and Daisuke Yoshioka and Joh Yonemura and Yoshio Kamiya and Takamitsu Ishizuka and Yoshihito Nakagawa and Mitsuo Nagasaka and Masami Iwata and Tomiyasu Arisawa and Ichiro Hirata",
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Okubo, M, Tahara, T, Shibata, T, Yamashita, H, Nakamura, M, Yoshioka, D, Yonemura, J, Kamiya, Y, Ishizuka, T, Nakagawa, Y, Nagasaka, M, Iwata, M, Arisawa, T & Hirata, I 2010, 'Association between common genetic variants in pre-microRNAs and the clinicopathological characteristics and survival of gastric cancer patients', Experimental and Therapeutic Medicine, vol. 1, no. 6, pp. 1035-1040. https://doi.org/10.3892/etm.2010.154

Association between common genetic variants in pre-microRNAs and the clinicopathological characteristics and survival of gastric cancer patients. / Okubo, Masaaki; Tahara, Tomomitsu; Shibata, Tomoyuki; Yamashita, Hiromi; Nakamura, Masakatsu; Yoshioka, Daisuke; Yonemura, Joh; Kamiya, Yoshio; Ishizuka, Takamitsu; Nakagawa, Yoshihito; Nagasaka, Mitsuo; Iwata, Masami; Arisawa, Tomiyasu; Hirata, Ichiro.

In: Experimental and Therapeutic Medicine, Vol. 1, No. 6, 01.11.2010, p. 1035-1040.

Research output: Contribution to journalArticle

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T1 - Association between common genetic variants in pre-microRNAs and the clinicopathological characteristics and survival of gastric cancer patients

AU - Okubo, Masaaki

AU - Tahara, Tomomitsu

AU - Shibata, Tomoyuki

AU - Yamashita, Hiromi

AU - Nakamura, Masakatsu

AU - Yoshioka, Daisuke

AU - Yonemura, Joh

AU - Kamiya, Yoshio

AU - Ishizuka, Takamitsu

AU - Nakagawa, Yoshihito

AU - Nagasaka, Mitsuo

AU - Iwata, Masami

AU - Arisawa, Tomiyasu

AU - Hirata, Ichiro

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Common single-nucleotide polymorphisms (SNPs) in micrornas (miRNAs) have been shown to be associated with susceptibility to several types of human cancer. We evaluated the association between three SNPs (rs11614913, rs2910164 and rs3746444) in pre-miRNAs (mir-196a2, mir-146a and mir-499) and various clinicopathological characteristics, including CpG island hypermethylation (CIHM) status and overall survival in gastric cancer (GC) patients. rs11614913 (T>C), rs2910164 (C>G) and rs3746444 (A>G) SNPs were genotyped in 127 Gc patients. cihm of p14, p16, dap-kinase and cdh1 genes was determined by methylation-specific polymerase chain reaction in the cancer tissues. A significant marginal association was found between the rs11614913 CC genotype and polypoid or elevated type morphology in early-stage GC (Or=6.29, 95% CI 1.18-33.47, p=0.03). the rs2910164 CC and CG genotypes were associated with increased susceptibility to cihm of dap-kinase (CC+CG, OR=5.48, 95% CI 1.30-23.10, p=0.02; CC, OR=6.93, 95% CI 1.37-35.02, P=0.02; CG, OR=4.24, 95% CI 0.87-20.78, p=0.07). the 11614913 TT and TC genotypes were associated with a higher number of CIHM (no. of CIHM 0-1 vs. 2-4; TT+TC, OR=3.67, 95% CI 0.98-13.72, p=0.05; TC, OR=4.08, 95% CI 1.04-15.97, p=0.04). When the subjects were divided according to age group, the combined rs11614913 TT+TC genotype tended to be associated with worse overall survival than the CC genotype in patients younger than 65 years of age (p=0.05). The combined rs2910164 CG+GG genotype also tended to be associated with worse overall survival than the CC genotype in the same age group (p=0.09). It appears that rs11614913 and rs2910164 SNPNPs in pre-miRNAs (miR-196a2 and miR-146a) affect the clinicopathological characteristics of GC, including its morphological appearance, CIHM status and overall survival.

AB - Common single-nucleotide polymorphisms (SNPs) in micrornas (miRNAs) have been shown to be associated with susceptibility to several types of human cancer. We evaluated the association between three SNPs (rs11614913, rs2910164 and rs3746444) in pre-miRNAs (mir-196a2, mir-146a and mir-499) and various clinicopathological characteristics, including CpG island hypermethylation (CIHM) status and overall survival in gastric cancer (GC) patients. rs11614913 (T>C), rs2910164 (C>G) and rs3746444 (A>G) SNPs were genotyped in 127 Gc patients. cihm of p14, p16, dap-kinase and cdh1 genes was determined by methylation-specific polymerase chain reaction in the cancer tissues. A significant marginal association was found between the rs11614913 CC genotype and polypoid or elevated type morphology in early-stage GC (Or=6.29, 95% CI 1.18-33.47, p=0.03). the rs2910164 CC and CG genotypes were associated with increased susceptibility to cihm of dap-kinase (CC+CG, OR=5.48, 95% CI 1.30-23.10, p=0.02; CC, OR=6.93, 95% CI 1.37-35.02, P=0.02; CG, OR=4.24, 95% CI 0.87-20.78, p=0.07). the 11614913 TT and TC genotypes were associated with a higher number of CIHM (no. of CIHM 0-1 vs. 2-4; TT+TC, OR=3.67, 95% CI 0.98-13.72, p=0.05; TC, OR=4.08, 95% CI 1.04-15.97, p=0.04). When the subjects were divided according to age group, the combined rs11614913 TT+TC genotype tended to be associated with worse overall survival than the CC genotype in patients younger than 65 years of age (p=0.05). The combined rs2910164 CG+GG genotype also tended to be associated with worse overall survival than the CC genotype in the same age group (p=0.09). It appears that rs11614913 and rs2910164 SNPNPs in pre-miRNAs (miR-196a2 and miR-146a) affect the clinicopathological characteristics of GC, including its morphological appearance, CIHM status and overall survival.

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