Association between gene polymorphisms of SLC22A3 and methamphetamine use disorder

Nagisa Aoyama, Nagahide Takahashi, Kiyoyuki Kitaichi, Ryoko Ishihara, Shinichi Saito, Nobuhisa Maeno, Xiaofei Ji, Kenji Takagi, Yoshimoto Sekine, Masaomi Iyo, Mutsuo Harano, Tokutaro Komiyama, Mitsuhiko Yamada, Ichiro Sora, Hiroshi Ujike, Nakao Iwata, Toshiya Inada, Norio Ozaki

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)


Background: Methamphetamine (MAP) is one of the most frequently used illegal substances in Japan, and family and twin studies have suggested that genetic factors contribute to psychostimulant dependence, including MAP dependence. Organic cation transporter 3 (OCT3) has been reported to be involved in the disposition of MAP as well as MAP-induced behavioral changes in animals. Moreover, SLC22A3 (which encodes OCT3) is a candidate gene for MAP dependence because it is located within a chromosomal region associated with substance dependence. Methods: Using 96 healthy control subjects, linkage disequilibrium (LD) within the SLC22A3 was investigated, and 5 single-nucleotide polymorphisms (SNPs) were selected as haplotype tag SNPs to search for an association with MAP dependence. Single-marker analyses and haplotype analyses of these SNPs were performed in 213 subjects with MAP dependence and 443 healthy controls. Results: SLC22A3 polymorphisms were not significantly associated with MAP dependence in any of the single-marker and haplotype analyses. When subjects with MAP dependence were divided into polysubstance and single-MAP users, genotype and allele frequency of SNP2 (p=0.024, p=0.011, respectively), allele frequency of SNP3 (p=0.037), and haplotypic frequencies for these 2 SNPs (p=0.0438) differed significantly between groups. Conclusions: These results suggest that polymorphisms of SLC22A3 are related to the development of polysubstance use in Japanese patients with MAP dependence.

Original languageEnglish
Pages (from-to)1644-1649
Number of pages6
JournalAlcoholism: Clinical and Experimental Research
Issue number10
Publication statusPublished - 10-2006

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health


Dive into the research topics of 'Association between gene polymorphisms of SLC22A3 and methamphetamine use disorder'. Together they form a unique fingerprint.

Cite this