Association between serum levels of carotenoids and serum asymmetric dimethylarginine levels in Japanese subjects

Rika Watarai, Koji Suzuki, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Hiroya Yamada, Takeshi Hamajima, Nobuyuki Hamajima, Takashi Inoue

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of endothelium nitric oxide synthase (NOS). ADMA binds to a substrate-binding site of NOS and then inhibits nitric oxide production from vascular endothelial cells. Elevated ADMA levels are a risk factor for cardiovascular disease. Recently, it was reported that plasma ADMA levels were negatively correlated with vegetable and fruit consumption. The purpose of this study was to examine the association between serum levels of carotenoids and serum ADMA levels in Japanese subjects. Methods: We conducted a cross-sectional study of 470 subjects (203 men and 267 women) who attended a health examination in August 2011. Serum levels of several carotenoids were separately measured by high-performance liquid chromatography. Serum ADMA levels were determined by using an enzyme-linked immunosorbent assay kit. Results: In women, the multivariate-adjusted odds ratios (ORs) of elevated serum ADMA levels were significantly decreased in the highest tertile for β-cryptoxanthin (OR 0.47, 95% CI 0.23-0.95), α-carotene (OR 0.39, 95% CI 0.18-0.79), and β-carotene (OR 0.36, 95% CI 0.17-0.73) compared to the lowest tertile. In men, significantly decreased ORs were observed in the highest tertiles of serum zeaxanthin/lutein (OR 0.23, 95% CI 0.06-0.69) and a-carotene (OR 0.26, 95% CI 0.07-0.82), and in the middle and the highest tertiles of serum β-carotene (OR 0.27, 95% CI 0.09-0.74 and OR 0.20, 95% CI 0.03-0.88, respectively) when the tertile cutoff points of women were extrapolated to men. Conclusions: Higher serum levels of carotenoids, such as α-carotene and β-carotene, may help to prevent elevated serum ADMA levels in Japanese subjects.

Original languageEnglish
Pages (from-to)250-257
Number of pages8
JournalJournal of epidemiology
Volume24
Issue number3
DOIs
Publication statusPublished - 01-01-2014

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Carotenoids
Odds Ratio
Serum
Nitric Oxide Synthase
N,N-dimethylarginine
Lutein
Vegetables
Endothelium
Fruit
Nitric Oxide
Cardiovascular Diseases
Endothelial Cells
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Binding Sites
High Pressure Liquid Chromatography
Health

All Science Journal Classification (ASJC) codes

  • Epidemiology

Cite this

@article{594789a27c8045c985dab9ff41275458,
title = "Association between serum levels of carotenoids and serum asymmetric dimethylarginine levels in Japanese subjects",
abstract = "Background: Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of endothelium nitric oxide synthase (NOS). ADMA binds to a substrate-binding site of NOS and then inhibits nitric oxide production from vascular endothelial cells. Elevated ADMA levels are a risk factor for cardiovascular disease. Recently, it was reported that plasma ADMA levels were negatively correlated with vegetable and fruit consumption. The purpose of this study was to examine the association between serum levels of carotenoids and serum ADMA levels in Japanese subjects. Methods: We conducted a cross-sectional study of 470 subjects (203 men and 267 women) who attended a health examination in August 2011. Serum levels of several carotenoids were separately measured by high-performance liquid chromatography. Serum ADMA levels were determined by using an enzyme-linked immunosorbent assay kit. Results: In women, the multivariate-adjusted odds ratios (ORs) of elevated serum ADMA levels were significantly decreased in the highest tertile for β-cryptoxanthin (OR 0.47, 95{\%} CI 0.23-0.95), α-carotene (OR 0.39, 95{\%} CI 0.18-0.79), and β-carotene (OR 0.36, 95{\%} CI 0.17-0.73) compared to the lowest tertile. In men, significantly decreased ORs were observed in the highest tertiles of serum zeaxanthin/lutein (OR 0.23, 95{\%} CI 0.06-0.69) and a-carotene (OR 0.26, 95{\%} CI 0.07-0.82), and in the middle and the highest tertiles of serum β-carotene (OR 0.27, 95{\%} CI 0.09-0.74 and OR 0.20, 95{\%} CI 0.03-0.88, respectively) when the tertile cutoff points of women were extrapolated to men. Conclusions: Higher serum levels of carotenoids, such as α-carotene and β-carotene, may help to prevent elevated serum ADMA levels in Japanese subjects.",
author = "Rika Watarai and Koji Suzuki and Naohiro Ichino and Keisuke Osakabe and Keiko Sugimoto and Hiroya Yamada and Takeshi Hamajima and Nobuyuki Hamajima and Takashi Inoue",
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language = "English",
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Association between serum levels of carotenoids and serum asymmetric dimethylarginine levels in Japanese subjects. / Watarai, Rika; Suzuki, Koji; Ichino, Naohiro; Osakabe, Keisuke; Sugimoto, Keiko; Yamada, Hiroya; Hamajima, Takeshi; Hamajima, Nobuyuki; Inoue, Takashi.

In: Journal of epidemiology, Vol. 24, No. 3, 01.01.2014, p. 250-257.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Association between serum levels of carotenoids and serum asymmetric dimethylarginine levels in Japanese subjects

AU - Watarai, Rika

AU - Suzuki, Koji

AU - Ichino, Naohiro

AU - Osakabe, Keisuke

AU - Sugimoto, Keiko

AU - Yamada, Hiroya

AU - Hamajima, Takeshi

AU - Hamajima, Nobuyuki

AU - Inoue, Takashi

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of endothelium nitric oxide synthase (NOS). ADMA binds to a substrate-binding site of NOS and then inhibits nitric oxide production from vascular endothelial cells. Elevated ADMA levels are a risk factor for cardiovascular disease. Recently, it was reported that plasma ADMA levels were negatively correlated with vegetable and fruit consumption. The purpose of this study was to examine the association between serum levels of carotenoids and serum ADMA levels in Japanese subjects. Methods: We conducted a cross-sectional study of 470 subjects (203 men and 267 women) who attended a health examination in August 2011. Serum levels of several carotenoids were separately measured by high-performance liquid chromatography. Serum ADMA levels were determined by using an enzyme-linked immunosorbent assay kit. Results: In women, the multivariate-adjusted odds ratios (ORs) of elevated serum ADMA levels were significantly decreased in the highest tertile for β-cryptoxanthin (OR 0.47, 95% CI 0.23-0.95), α-carotene (OR 0.39, 95% CI 0.18-0.79), and β-carotene (OR 0.36, 95% CI 0.17-0.73) compared to the lowest tertile. In men, significantly decreased ORs were observed in the highest tertiles of serum zeaxanthin/lutein (OR 0.23, 95% CI 0.06-0.69) and a-carotene (OR 0.26, 95% CI 0.07-0.82), and in the middle and the highest tertiles of serum β-carotene (OR 0.27, 95% CI 0.09-0.74 and OR 0.20, 95% CI 0.03-0.88, respectively) when the tertile cutoff points of women were extrapolated to men. Conclusions: Higher serum levels of carotenoids, such as α-carotene and β-carotene, may help to prevent elevated serum ADMA levels in Japanese subjects.

AB - Background: Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of endothelium nitric oxide synthase (NOS). ADMA binds to a substrate-binding site of NOS and then inhibits nitric oxide production from vascular endothelial cells. Elevated ADMA levels are a risk factor for cardiovascular disease. Recently, it was reported that plasma ADMA levels were negatively correlated with vegetable and fruit consumption. The purpose of this study was to examine the association between serum levels of carotenoids and serum ADMA levels in Japanese subjects. Methods: We conducted a cross-sectional study of 470 subjects (203 men and 267 women) who attended a health examination in August 2011. Serum levels of several carotenoids were separately measured by high-performance liquid chromatography. Serum ADMA levels were determined by using an enzyme-linked immunosorbent assay kit. Results: In women, the multivariate-adjusted odds ratios (ORs) of elevated serum ADMA levels were significantly decreased in the highest tertile for β-cryptoxanthin (OR 0.47, 95% CI 0.23-0.95), α-carotene (OR 0.39, 95% CI 0.18-0.79), and β-carotene (OR 0.36, 95% CI 0.17-0.73) compared to the lowest tertile. In men, significantly decreased ORs were observed in the highest tertiles of serum zeaxanthin/lutein (OR 0.23, 95% CI 0.06-0.69) and a-carotene (OR 0.26, 95% CI 0.07-0.82), and in the middle and the highest tertiles of serum β-carotene (OR 0.27, 95% CI 0.09-0.74 and OR 0.20, 95% CI 0.03-0.88, respectively) when the tertile cutoff points of women were extrapolated to men. Conclusions: Higher serum levels of carotenoids, such as α-carotene and β-carotene, may help to prevent elevated serum ADMA levels in Japanese subjects.

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