Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility

Suyoun Chung, Hidewaki Nakagawa, Motohide Uemura, Lianhua Piao, Kyota Ashikawa, Naoya Hosono, Ryo Takata, Shusuke Akamatsu, Takahisa Kawaguchi, Takashi Morizono, Tatsuhiko Tsunoda, Yataro Daigo, Koichi Matsuda, Naoyuki Kamatani, Yusuke Nakamura, Michiaki Kubo

Research output: Contribution to journalArticle

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Abstract

Recent genome-wide association studies reported strong and reproducible associations of multiple genetic variants in a large "gene-desert" region of chromosome 8q24 with susceptibility to prostate cancer (PC). However, the causative or functional variants of these 8q24 loci and their biological mechanisms associated with PC susceptibility remain unclear and should be investigated. Here, focusing on its most centromeric region (so-called Region 2: Chr8: 128.14-128.28 Mb) among the multiple PC loci on 8q24, we performed fine mapping and re-sequencing of this critical region and identified SNPs (single nucleotide polymorphisms) between rs1456315 and rs7463708 (chr8: 128,173,119-128,173,237 bp) to be most significantly associated with PC susceptibility (P = 2.00 × 10-24, OR = 1.74, 95% confidence interval = 1.56-1.93). Importantly, we show that this region was transcribed as a 13 kb intron-less long non-coding RNA (ncRNA), termed PRNCR1 (prostate cancer non-coding RNA 1), and PRNCR1 expression was upregulated in some of the PC cells as well as precursor lesion prostatic intraepithelial neoplasia. Knockdown of PRNCR1 by siRNA attenuated the viability of PC cells and the transactivation activity of androgen receptor, which indicates that PRNCR1 could be involved in prostate carcinogenesis possibly through androgen receptor activity. These findings could provide a new insight in understanding the pathogenesis of genetic factors for PC susceptibility and prostate carcinogenesis.

Original languageEnglish
Pages (from-to)245-252
Number of pages8
JournalCancer Science
Volume102
Issue number1
DOIs
Publication statusPublished - 01-01-2011

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Long Noncoding RNA
Prostatic Neoplasms
Untranslated RNA
Androgen Receptors
Carcinogenesis
Prostatic Intraepithelial Neoplasia
Genome-Wide Association Study
Introns
Small Interfering RNA
Transcriptional Activation
Single Nucleotide Polymorphism

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Chung, Suyoun ; Nakagawa, Hidewaki ; Uemura, Motohide ; Piao, Lianhua ; Ashikawa, Kyota ; Hosono, Naoya ; Takata, Ryo ; Akamatsu, Shusuke ; Kawaguchi, Takahisa ; Morizono, Takashi ; Tsunoda, Tatsuhiko ; Daigo, Yataro ; Matsuda, Koichi ; Kamatani, Naoyuki ; Nakamura, Yusuke ; Kubo, Michiaki. / Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility. In: Cancer Science. 2011 ; Vol. 102, No. 1. pp. 245-252.
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abstract = "Recent genome-wide association studies reported strong and reproducible associations of multiple genetic variants in a large {"}gene-desert{"} region of chromosome 8q24 with susceptibility to prostate cancer (PC). However, the causative or functional variants of these 8q24 loci and their biological mechanisms associated with PC susceptibility remain unclear and should be investigated. Here, focusing on its most centromeric region (so-called Region 2: Chr8: 128.14-128.28 Mb) among the multiple PC loci on 8q24, we performed fine mapping and re-sequencing of this critical region and identified SNPs (single nucleotide polymorphisms) between rs1456315 and rs7463708 (chr8: 128,173,119-128,173,237 bp) to be most significantly associated with PC susceptibility (P = 2.00 × 10-24, OR = 1.74, 95{\%} confidence interval = 1.56-1.93). Importantly, we show that this region was transcribed as a 13 kb intron-less long non-coding RNA (ncRNA), termed PRNCR1 (prostate cancer non-coding RNA 1), and PRNCR1 expression was upregulated in some of the PC cells as well as precursor lesion prostatic intraepithelial neoplasia. Knockdown of PRNCR1 by siRNA attenuated the viability of PC cells and the transactivation activity of androgen receptor, which indicates that PRNCR1 could be involved in prostate carcinogenesis possibly through androgen receptor activity. These findings could provide a new insight in understanding the pathogenesis of genetic factors for PC susceptibility and prostate carcinogenesis.",
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Chung, S, Nakagawa, H, Uemura, M, Piao, L, Ashikawa, K, Hosono, N, Takata, R, Akamatsu, S, Kawaguchi, T, Morizono, T, Tsunoda, T, Daigo, Y, Matsuda, K, Kamatani, N, Nakamura, Y & Kubo, M 2011, 'Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility', Cancer Science, vol. 102, no. 1, pp. 245-252. https://doi.org/10.1111/j.1349-7006.2010.01737.x

Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility. / Chung, Suyoun; Nakagawa, Hidewaki; Uemura, Motohide; Piao, Lianhua; Ashikawa, Kyota; Hosono, Naoya; Takata, Ryo; Akamatsu, Shusuke; Kawaguchi, Takahisa; Morizono, Takashi; Tsunoda, Tatsuhiko; Daigo, Yataro; Matsuda, Koichi; Kamatani, Naoyuki; Nakamura, Yusuke; Kubo, Michiaki.

In: Cancer Science, Vol. 102, No. 1, 01.01.2011, p. 245-252.

Research output: Contribution to journalArticle

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AU - Chung, Suyoun

AU - Nakagawa, Hidewaki

AU - Uemura, Motohide

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AU - Ashikawa, Kyota

AU - Hosono, Naoya

AU - Takata, Ryo

AU - Akamatsu, Shusuke

AU - Kawaguchi, Takahisa

AU - Morizono, Takashi

AU - Tsunoda, Tatsuhiko

AU - Daigo, Yataro

AU - Matsuda, Koichi

AU - Kamatani, Naoyuki

AU - Nakamura, Yusuke

AU - Kubo, Michiaki

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