Association of circulating miR-20a, miR-27a, and miR-126 with non-alcoholic fatty liver disease in general population

Yoshitaka Ando, Mirai Yamazaki, Hiroya Yamada, Eiji Munetsuna, Ryosuke Fujii, Genki Mizuno, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Hiroaki Ishikawa, Koji Ohashi, Ryoji Teradaira, Yoshiji Ohta, Nobuyuki Hamajima, Shuji Hashimoto, Koji Suzuki

Research output: Contribution to journalArticle

Abstract

Non-alcoholic fatty liver disease (NAFLD) is closely associated with obesity, metabolic syndrome, and type II diabetes mellitus. Recently, circulating microRNAs (miRNAs) have been proposed as useful disease biomarkers. We examined whether circulating miRNAs, such as miR-20a, miR-27a, and miR-126, were useful biomarkers for NAFLD. We conducted a cross-sectional analysis of 527 subjects aged 39 years or older who had undergone a health examination in the Yakumo Study. Of the residents, 92 were diagnosed with NAFLD using a registered medical sonographer. Serum miR-20a, miR-27a and miR-126 levels were measured by quantitative real-time PCR. We then calculated the odds ratios for serum miRNA level changes according to the severity of NAFLD using normal liver status as the reference group. Serum levels of miR-20a and 27a, but not miR-126, were significantly lower in NAFLD subjects than normal subjects. Serum miR-20a and miR-27a levels were significantly lower in both male and female severe NAFLD subjects. Logistic regression analysis showed a significant relationship between low circulating miR-20a and 27a levels and severe NAFLD. Down-regulated circulating miR-20a and 27a levels were significantly associated with severe NAFLD in the general population. Circulating miR-20a and miR-27a may be useful biomarkers for severe NAFLD.

Original languageEnglish
Article number18856
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 01-12-2019

Fingerprint

Population
MicroRNAs
Biomarkers
Serum
Non-alcoholic Fatty Liver Disease
Type 2 Diabetes Mellitus
Real-Time Polymerase Chain Reaction
Obesity
Cross-Sectional Studies
Logistic Models
Odds Ratio
Regression Analysis
Liver
Health

All Science Journal Classification (ASJC) codes

  • General

Cite this

Ando, Yoshitaka ; Yamazaki, Mirai ; Yamada, Hiroya ; Munetsuna, Eiji ; Fujii, Ryosuke ; Mizuno, Genki ; Ichino, Naohiro ; Osakabe, Keisuke ; Sugimoto, Keiko ; Ishikawa, Hiroaki ; Ohashi, Koji ; Teradaira, Ryoji ; Ohta, Yoshiji ; Hamajima, Nobuyuki ; Hashimoto, Shuji ; Suzuki, Koji. / Association of circulating miR-20a, miR-27a, and miR-126 with non-alcoholic fatty liver disease in general population. In: Scientific reports. 2019 ; Vol. 9, No. 1.
@article{2afe17d76ba041f1ab0c3b6913e70020,
title = "Association of circulating miR-20a, miR-27a, and miR-126 with non-alcoholic fatty liver disease in general population",
abstract = "Non-alcoholic fatty liver disease (NAFLD) is closely associated with obesity, metabolic syndrome, and type II diabetes mellitus. Recently, circulating microRNAs (miRNAs) have been proposed as useful disease biomarkers. We examined whether circulating miRNAs, such as miR-20a, miR-27a, and miR-126, were useful biomarkers for NAFLD. We conducted a cross-sectional analysis of 527 subjects aged 39 years or older who had undergone a health examination in the Yakumo Study. Of the residents, 92 were diagnosed with NAFLD using a registered medical sonographer. Serum miR-20a, miR-27a and miR-126 levels were measured by quantitative real-time PCR. We then calculated the odds ratios for serum miRNA level changes according to the severity of NAFLD using normal liver status as the reference group. Serum levels of miR-20a and 27a, but not miR-126, were significantly lower in NAFLD subjects than normal subjects. Serum miR-20a and miR-27a levels were significantly lower in both male and female severe NAFLD subjects. Logistic regression analysis showed a significant relationship between low circulating miR-20a and 27a levels and severe NAFLD. Down-regulated circulating miR-20a and 27a levels were significantly associated with severe NAFLD in the general population. Circulating miR-20a and miR-27a may be useful biomarkers for severe NAFLD.",
author = "Yoshitaka Ando and Mirai Yamazaki and Hiroya Yamada and Eiji Munetsuna and Ryosuke Fujii and Genki Mizuno and Naohiro Ichino and Keisuke Osakabe and Keiko Sugimoto and Hiroaki Ishikawa and Koji Ohashi and Ryoji Teradaira and Yoshiji Ohta and Nobuyuki Hamajima and Shuji Hashimoto and Koji Suzuki",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41598-019-55076-z",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

Ando, Y, Yamazaki, M, Yamada, H, Munetsuna, E, Fujii, R, Mizuno, G, Ichino, N, Osakabe, K, Sugimoto, K, Ishikawa, H, Ohashi, K, Teradaira, R, Ohta, Y, Hamajima, N, Hashimoto, S & Suzuki, K 2019, 'Association of circulating miR-20a, miR-27a, and miR-126 with non-alcoholic fatty liver disease in general population', Scientific reports, vol. 9, no. 1, 18856. https://doi.org/10.1038/s41598-019-55076-z

Association of circulating miR-20a, miR-27a, and miR-126 with non-alcoholic fatty liver disease in general population. / Ando, Yoshitaka; Yamazaki, Mirai; Yamada, Hiroya; Munetsuna, Eiji; Fujii, Ryosuke; Mizuno, Genki; Ichino, Naohiro; Osakabe, Keisuke; Sugimoto, Keiko; Ishikawa, Hiroaki; Ohashi, Koji; Teradaira, Ryoji; Ohta, Yoshiji; Hamajima, Nobuyuki; Hashimoto, Shuji; Suzuki, Koji.

In: Scientific reports, Vol. 9, No. 1, 18856, 01.12.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Association of circulating miR-20a, miR-27a, and miR-126 with non-alcoholic fatty liver disease in general population

AU - Ando, Yoshitaka

AU - Yamazaki, Mirai

AU - Yamada, Hiroya

AU - Munetsuna, Eiji

AU - Fujii, Ryosuke

AU - Mizuno, Genki

AU - Ichino, Naohiro

AU - Osakabe, Keisuke

AU - Sugimoto, Keiko

AU - Ishikawa, Hiroaki

AU - Ohashi, Koji

AU - Teradaira, Ryoji

AU - Ohta, Yoshiji

AU - Hamajima, Nobuyuki

AU - Hashimoto, Shuji

AU - Suzuki, Koji

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Non-alcoholic fatty liver disease (NAFLD) is closely associated with obesity, metabolic syndrome, and type II diabetes mellitus. Recently, circulating microRNAs (miRNAs) have been proposed as useful disease biomarkers. We examined whether circulating miRNAs, such as miR-20a, miR-27a, and miR-126, were useful biomarkers for NAFLD. We conducted a cross-sectional analysis of 527 subjects aged 39 years or older who had undergone a health examination in the Yakumo Study. Of the residents, 92 were diagnosed with NAFLD using a registered medical sonographer. Serum miR-20a, miR-27a and miR-126 levels were measured by quantitative real-time PCR. We then calculated the odds ratios for serum miRNA level changes according to the severity of NAFLD using normal liver status as the reference group. Serum levels of miR-20a and 27a, but not miR-126, were significantly lower in NAFLD subjects than normal subjects. Serum miR-20a and miR-27a levels were significantly lower in both male and female severe NAFLD subjects. Logistic regression analysis showed a significant relationship between low circulating miR-20a and 27a levels and severe NAFLD. Down-regulated circulating miR-20a and 27a levels were significantly associated with severe NAFLD in the general population. Circulating miR-20a and miR-27a may be useful biomarkers for severe NAFLD.

AB - Non-alcoholic fatty liver disease (NAFLD) is closely associated with obesity, metabolic syndrome, and type II diabetes mellitus. Recently, circulating microRNAs (miRNAs) have been proposed as useful disease biomarkers. We examined whether circulating miRNAs, such as miR-20a, miR-27a, and miR-126, were useful biomarkers for NAFLD. We conducted a cross-sectional analysis of 527 subjects aged 39 years or older who had undergone a health examination in the Yakumo Study. Of the residents, 92 were diagnosed with NAFLD using a registered medical sonographer. Serum miR-20a, miR-27a and miR-126 levels were measured by quantitative real-time PCR. We then calculated the odds ratios for serum miRNA level changes according to the severity of NAFLD using normal liver status as the reference group. Serum levels of miR-20a and 27a, but not miR-126, were significantly lower in NAFLD subjects than normal subjects. Serum miR-20a and miR-27a levels were significantly lower in both male and female severe NAFLD subjects. Logistic regression analysis showed a significant relationship between low circulating miR-20a and 27a levels and severe NAFLD. Down-regulated circulating miR-20a and 27a levels were significantly associated with severe NAFLD in the general population. Circulating miR-20a and miR-27a may be useful biomarkers for severe NAFLD.

UR - http://www.scopus.com/inward/record.url?scp=85076408005&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85076408005&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-55076-z

DO - 10.1038/s41598-019-55076-z

M3 - Article

C2 - 31827150

AN - SCOPUS:85076408005

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 18856

ER -