TY - JOUR
T1 - Association of hepatitis B virus subgenotypes and basal core promoter/ precore region variants with the clinical features of patients with acute hepatitis
AU - Hayashi, Kazuhiko
AU - Katano, Yoshiaki
AU - Takeda, Yasushi
AU - Honda, Takashi
AU - Ishigami, Masatoshi
AU - Itoh, Akihiro
AU - Hirooka, Yoshiki
AU - Nakano, Isao
AU - Yoshioka, Kentaro
AU - Toyoda, Hidenori
AU - Kumada, Takashi
AU - Goto, Hidemi
PY - 2008/7
Y1 - 2008/7
N2 - Background: In endemic areas, including Japan, basal core promoter (BCP) and precore (PC) variants of hepatitis B virus (HBV) have been reported to be associated with the clinical outcome of acute hepatitis B patients. However, the associations of BCP/PC variants with clinical outcomes have not been observed in nonendemic areas. HBV subgenotypes, which show geographic variations in prevalence, may underlie this discrepancy in clinical outcomes. Little is known about the differences in the clinical and virological features of HBV subgenotypes and BCP/PC variants. The aim of this study was to investigate the distributions of subgenotypes and BCP/PC variants to identify clinical differences in acute hepatitis B patients. Methods: One hundred thirty-nine patients with acute hepatitis were enrolled. Nested polymerase chain reaction was used to amplify the pre-S region of HBV for genotyping and the BCP/PC regions for variant screening. Results: HBV subgenotypes A1 (n = 3), A2 (n = 28), B1 (n = 3), B2 (n = 9), C1 (n = 5), C2 (n = 84), C variant (n = 1), D2 (n = 3), and H (n = 3) were detected. BCP/PC variants were not associated with progression to chronic hepatitis. Patients infected with subgenotype C2 who progressed to fulminant hepatic failure frequently carried variants at nucleotides non-T1753 and non-T1754 and T1762, A1764, and A1896. Conclusions: BCP/PC variants would be associated with progression to fulminant hepatitis in subgenotype C2. Knowledge of HBV subgenotypes and BCP/PC variants is useful for developing strategies to treat acute hepatitis B patients.
AB - Background: In endemic areas, including Japan, basal core promoter (BCP) and precore (PC) variants of hepatitis B virus (HBV) have been reported to be associated with the clinical outcome of acute hepatitis B patients. However, the associations of BCP/PC variants with clinical outcomes have not been observed in nonendemic areas. HBV subgenotypes, which show geographic variations in prevalence, may underlie this discrepancy in clinical outcomes. Little is known about the differences in the clinical and virological features of HBV subgenotypes and BCP/PC variants. The aim of this study was to investigate the distributions of subgenotypes and BCP/PC variants to identify clinical differences in acute hepatitis B patients. Methods: One hundred thirty-nine patients with acute hepatitis were enrolled. Nested polymerase chain reaction was used to amplify the pre-S region of HBV for genotyping and the BCP/PC regions for variant screening. Results: HBV subgenotypes A1 (n = 3), A2 (n = 28), B1 (n = 3), B2 (n = 9), C1 (n = 5), C2 (n = 84), C variant (n = 1), D2 (n = 3), and H (n = 3) were detected. BCP/PC variants were not associated with progression to chronic hepatitis. Patients infected with subgenotype C2 who progressed to fulminant hepatic failure frequently carried variants at nucleotides non-T1753 and non-T1754 and T1762, A1764, and A1896. Conclusions: BCP/PC variants would be associated with progression to fulminant hepatitis in subgenotype C2. Knowledge of HBV subgenotypes and BCP/PC variants is useful for developing strategies to treat acute hepatitis B patients.
UR - http://www.scopus.com/inward/record.url?scp=48449096610&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48449096610&partnerID=8YFLogxK
U2 - 10.1007/s00535-008-2197-2
DO - 10.1007/s00535-008-2197-2
M3 - Article
C2 - 18648743
AN - SCOPUS:48449096610
SN - 0944-1174
VL - 43
SP - 558
EP - 564
JO - Journal of Gastroenterology
JF - Journal of Gastroenterology
IS - 7
ER -