Association of MR1 protein, an MHC class I-related molecule, with β2-microglobulin

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Abstract

MR1 is a major histocompatibility complex (MHC) class I-related gene conserved among mammals, and its predicted amino acid sequence is relatively closer to the classical MHC class I molecules among several divergent class I molecules. However, as its molecular nature and function have not yet been clarified, we set out in this study to establish transfected P388 murine cell lines that stably produce a large number of MR1 proteins and conducted analyses to investigate the molecular nature of MR1. Immunoprecipitation and Western blot analyses with specific antisera revealed that the MR1 protein can associate with β2-microglobulin, suggesting its molecular form of a typical class I heterodimer composed of a heavy and a light chain (β2-microglobulin), like the classical MHC class I molecules.

Original languageEnglish
Pages (from-to)722-729
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume290
Issue number2
DOIs
Publication statusPublished - 01-01-2002

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Major Histocompatibility Complex
Molecules
MHC Class I Genes
Proteins
Mammals
Immunoprecipitation
Immune Sera
Amino Acid Sequence
Genes
Western Blotting
Cells
Light
Amino Acids
Cell Line

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "MR1 is a major histocompatibility complex (MHC) class I-related gene conserved among mammals, and its predicted amino acid sequence is relatively closer to the classical MHC class I molecules among several divergent class I molecules. However, as its molecular nature and function have not yet been clarified, we set out in this study to establish transfected P388 murine cell lines that stably produce a large number of MR1 proteins and conducted analyses to investigate the molecular nature of MR1. Immunoprecipitation and Western blot analyses with specific antisera revealed that the MR1 protein can associate with β2-microglobulin, suggesting its molecular form of a typical class I heterodimer composed of a heavy and a light chain (β2-microglobulin), like the classical MHC class I molecules.",
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AB - MR1 is a major histocompatibility complex (MHC) class I-related gene conserved among mammals, and its predicted amino acid sequence is relatively closer to the classical MHC class I molecules among several divergent class I molecules. However, as its molecular nature and function have not yet been clarified, we set out in this study to establish transfected P388 murine cell lines that stably produce a large number of MR1 proteins and conducted analyses to investigate the molecular nature of MR1. Immunoprecipitation and Western blot analyses with specific antisera revealed that the MR1 protein can associate with β2-microglobulin, suggesting its molecular form of a typical class I heterodimer composed of a heavy and a light chain (β2-microglobulin), like the classical MHC class I molecules.

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