TY - JOUR
T1 - Association of MR1 protein, an MHC class I-related molecule, with β2-microglobulin
AU - Yamaguchi, Hisateru
AU - Hashimoto, Keiichiro
N1 - Funding Information:
We thank Dr. Y. Kurosawa for his general support in the present study. This work was supported by PROBRAIN and in part by grants from the Ministry of Education, Science, Sports, and Culture of Japan; Japan Society for the Promotion of Science; a Grant-in-Aid for Fujita Health University High-Tech Research Center from the Ministry of Education, Science, Sports, and Culture of Japan; and Fujita Health University.
PY - 2002
Y1 - 2002
N2 - MR1 is a major histocompatibility complex (MHC) class I-related gene conserved among mammals, and its predicted amino acid sequence is relatively closer to the classical MHC class I molecules among several divergent class I molecules. However, as its molecular nature and function have not yet been clarified, we set out in this study to establish transfected P388 murine cell lines that stably produce a large number of MR1 proteins and conducted analyses to investigate the molecular nature of MR1. Immunoprecipitation and Western blot analyses with specific antisera revealed that the MR1 protein can associate with β2-microglobulin, suggesting its molecular form of a typical class I heterodimer composed of a heavy and a light chain (β2-microglobulin), like the classical MHC class I molecules.
AB - MR1 is a major histocompatibility complex (MHC) class I-related gene conserved among mammals, and its predicted amino acid sequence is relatively closer to the classical MHC class I molecules among several divergent class I molecules. However, as its molecular nature and function have not yet been clarified, we set out in this study to establish transfected P388 murine cell lines that stably produce a large number of MR1 proteins and conducted analyses to investigate the molecular nature of MR1. Immunoprecipitation and Western blot analyses with specific antisera revealed that the MR1 protein can associate with β2-microglobulin, suggesting its molecular form of a typical class I heterodimer composed of a heavy and a light chain (β2-microglobulin), like the classical MHC class I molecules.
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U2 - 10.1006/bbrc.2001.6277
DO - 10.1006/bbrc.2001.6277
M3 - Article
C2 - 11785959
AN - SCOPUS:0036297768
VL - 290
SP - 722
EP - 729
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -