Association of polymorphism of the p22PHOX component of NADPH oxidase in gastroduodenal diseases in Japan

Tomomitsu Tahara, Tomoyuki Shibata, Fangyu Wang, Masakatsu Nakamura, Masaaki Okubo, Daisuke Yoshioka, Mikijyu Sakata, Hiroshi Nakano, Ichiro Hirata, Tomiyasu Arisawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective. Superoxide has been implicated in the pathogenesis of Helicobacter pylori-related diseases through inflammation. NADPH oxidase, a major source of superoxide generation, plays a critical role in H. pylori-related gastric inflammation. The aim of this study was to clarify the effect of the p22PHOX C242T polymorphism, an essential component of NADPH oxidase in the risk of gastroduodenal diseases, on the severity of H. pylori-induced gastritis in a Japanese population. Material and methods. The study comprised 436 patients attending the Endoscopy Center of Fujita Health University Hospital. The p22PHOX C242T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Gastritis scores of antral gastric mucosa were assessed according to the updated Sydney system. Results. The 436 patients represented 106 gastric ulcers (24.3%), 48 duodenal ulcers (11.0%), and 282 non-ulcer subjects (64.7%). No association was found between p22PHOX polymorphism and the risk of ulcer diseases compared to non-ulcer subjects. However, among H. pylori-positive subjects, the degree of intestinal metaplasia tended to be lower in 242T carriers aged more than 60 years (p=0.0488). The same allele also decreased the risk of developing a more severe intestinal metaplasia in H. pylori-positive female subjects (p=0.0441). Conclusions. Our data suggest that the p22PHOX 242T allele is associated with a reduced risk of developing a more severe intestinal metaplasia in subjects older than 60 years of age and in female subjects with H. pylori infection.

Original languageEnglish
Pages (from-to)296-300
Number of pages5
JournalScandinavian Journal of Gastroenterology
Volume44
Issue number3
DOIs
Publication statusPublished - 01-03-2009

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NADPH Oxidase
Helicobacter pylori
Japan
Metaplasia
Gastritis
Superoxides
Alleles
Inflammation
Helicobacter Infections
Stomach Ulcer
Duodenal Ulcer
Gastric Mucosa
Restriction Fragment Length Polymorphisms
Endoscopy
Ulcer
Stomach
Polymerase Chain Reaction
Health
Population

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Tahara, Tomomitsu ; Shibata, Tomoyuki ; Wang, Fangyu ; Nakamura, Masakatsu ; Okubo, Masaaki ; Yoshioka, Daisuke ; Sakata, Mikijyu ; Nakano, Hiroshi ; Hirata, Ichiro ; Arisawa, Tomiyasu. / Association of polymorphism of the p22PHOX component of NADPH oxidase in gastroduodenal diseases in Japan. In: Scandinavian Journal of Gastroenterology. 2009 ; Vol. 44, No. 3. pp. 296-300.
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abstract = "Objective. Superoxide has been implicated in the pathogenesis of Helicobacter pylori-related diseases through inflammation. NADPH oxidase, a major source of superoxide generation, plays a critical role in H. pylori-related gastric inflammation. The aim of this study was to clarify the effect of the p22PHOX C242T polymorphism, an essential component of NADPH oxidase in the risk of gastroduodenal diseases, on the severity of H. pylori-induced gastritis in a Japanese population. Material and methods. The study comprised 436 patients attending the Endoscopy Center of Fujita Health University Hospital. The p22PHOX C242T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Gastritis scores of antral gastric mucosa were assessed according to the updated Sydney system. Results. The 436 patients represented 106 gastric ulcers (24.3{\%}), 48 duodenal ulcers (11.0{\%}), and 282 non-ulcer subjects (64.7{\%}). No association was found between p22PHOX polymorphism and the risk of ulcer diseases compared to non-ulcer subjects. However, among H. pylori-positive subjects, the degree of intestinal metaplasia tended to be lower in 242T carriers aged more than 60 years (p=0.0488). The same allele also decreased the risk of developing a more severe intestinal metaplasia in H. pylori-positive female subjects (p=0.0441). Conclusions. Our data suggest that the p22PHOX 242T allele is associated with a reduced risk of developing a more severe intestinal metaplasia in subjects older than 60 years of age and in female subjects with H. pylori infection.",
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Tahara, T, Shibata, T, Wang, F, Nakamura, M, Okubo, M, Yoshioka, D, Sakata, M, Nakano, H, Hirata, I & Arisawa, T 2009, 'Association of polymorphism of the p22PHOX component of NADPH oxidase in gastroduodenal diseases in Japan', Scandinavian Journal of Gastroenterology, vol. 44, no. 3, pp. 296-300. https://doi.org/10.1080/00365520701702348

Association of polymorphism of the p22PHOX component of NADPH oxidase in gastroduodenal diseases in Japan. / Tahara, Tomomitsu; Shibata, Tomoyuki; Wang, Fangyu; Nakamura, Masakatsu; Okubo, Masaaki; Yoshioka, Daisuke; Sakata, Mikijyu; Nakano, Hiroshi; Hirata, Ichiro; Arisawa, Tomiyasu.

In: Scandinavian Journal of Gastroenterology, Vol. 44, No. 3, 01.03.2009, p. 296-300.

Research output: Contribution to journalArticle

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T1 - Association of polymorphism of the p22PHOX component of NADPH oxidase in gastroduodenal diseases in Japan

AU - Tahara, Tomomitsu

AU - Shibata, Tomoyuki

AU - Wang, Fangyu

AU - Nakamura, Masakatsu

AU - Okubo, Masaaki

AU - Yoshioka, Daisuke

AU - Sakata, Mikijyu

AU - Nakano, Hiroshi

AU - Hirata, Ichiro

AU - Arisawa, Tomiyasu

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Objective. Superoxide has been implicated in the pathogenesis of Helicobacter pylori-related diseases through inflammation. NADPH oxidase, a major source of superoxide generation, plays a critical role in H. pylori-related gastric inflammation. The aim of this study was to clarify the effect of the p22PHOX C242T polymorphism, an essential component of NADPH oxidase in the risk of gastroduodenal diseases, on the severity of H. pylori-induced gastritis in a Japanese population. Material and methods. The study comprised 436 patients attending the Endoscopy Center of Fujita Health University Hospital. The p22PHOX C242T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Gastritis scores of antral gastric mucosa were assessed according to the updated Sydney system. Results. The 436 patients represented 106 gastric ulcers (24.3%), 48 duodenal ulcers (11.0%), and 282 non-ulcer subjects (64.7%). No association was found between p22PHOX polymorphism and the risk of ulcer diseases compared to non-ulcer subjects. However, among H. pylori-positive subjects, the degree of intestinal metaplasia tended to be lower in 242T carriers aged more than 60 years (p=0.0488). The same allele also decreased the risk of developing a more severe intestinal metaplasia in H. pylori-positive female subjects (p=0.0441). Conclusions. Our data suggest that the p22PHOX 242T allele is associated with a reduced risk of developing a more severe intestinal metaplasia in subjects older than 60 years of age and in female subjects with H. pylori infection.

AB - Objective. Superoxide has been implicated in the pathogenesis of Helicobacter pylori-related diseases through inflammation. NADPH oxidase, a major source of superoxide generation, plays a critical role in H. pylori-related gastric inflammation. The aim of this study was to clarify the effect of the p22PHOX C242T polymorphism, an essential component of NADPH oxidase in the risk of gastroduodenal diseases, on the severity of H. pylori-induced gastritis in a Japanese population. Material and methods. The study comprised 436 patients attending the Endoscopy Center of Fujita Health University Hospital. The p22PHOX C242T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Gastritis scores of antral gastric mucosa were assessed according to the updated Sydney system. Results. The 436 patients represented 106 gastric ulcers (24.3%), 48 duodenal ulcers (11.0%), and 282 non-ulcer subjects (64.7%). No association was found between p22PHOX polymorphism and the risk of ulcer diseases compared to non-ulcer subjects. However, among H. pylori-positive subjects, the degree of intestinal metaplasia tended to be lower in 242T carriers aged more than 60 years (p=0.0488). The same allele also decreased the risk of developing a more severe intestinal metaplasia in H. pylori-positive female subjects (p=0.0441). Conclusions. Our data suggest that the p22PHOX 242T allele is associated with a reduced risk of developing a more severe intestinal metaplasia in subjects older than 60 years of age and in female subjects with H. pylori infection.

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