Association of Sf9 cell proliferating cell nuclear antigen with the DNA replication site of Autographa californica multicapsid nucleopolyhedrovirus

Satoko Iwahori, Motoko Ikeda, Michihiro Kobayashi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The accumulation of cellular proliferating cell nuclear antigen (PCNA) in the nucleus of Sf9 cells has been shown to increase upon infection with Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). Here, analysis by DNase I treatment and chromatin immunoprecipitation revealed that cellular PCNA in the nucleus of Sf9 cells bound AcMNPV DNA. Immunocytochemical analysis showed colocalization of Sf9 cell PCNA and AcMNPV DNA replication sites. Similar colocalization was also observed in BmN-4 cells infected with Bombyx mori NPV, which is inherently missing the pcna gene. The amount of cellular PCNA associated with viral DNA replication sites was greater in cells infected with pcna-defective AcMNPV mutants than in cells infected with wild-type AcMNPV. These results suggest that both cellular and viral PCNAs are involved in AcMNPV DNA replication and that pcna-defective AcMNPV mutants are able to substitute cellular PCNA for viral PCNA.

Original languageEnglish
Pages (from-to)2857-2862
Number of pages6
JournalJournal of General Virology
Volume85
Issue number10
DOIs
Publication statusPublished - 10-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology

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