Association of subcutaneous and visceral fat with circulating microRNAs in a middle-aged Japanese population

Eiji Munetsuna, Hiroya Yamada, Yoshitaka Ando, Mirai Yamazaki, Yoshiki Tsuboi, Mari Kondo, Genki Mizuno, Hiroaki Ishikawa, Keiko Sugimoto, Keisuke Osakabe, Naohiro Ichino, Koji Ohashi, Nobuyuki Hamajima, Koji Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: It has been demonstrated that circulating microRNA profiles are affected by physiological conditions. Several studies have demonstrated that microRNAs play important roles in the regulation of adiposity. However, few have investigated the relationship between circulating microRNAs and obesity, which has become a major public health problem worldwide. This study investigated the association between circulating microRNAs and obesity in a Japanese population. Methods: Obesity parameters, such as subcutaneous and visceral fat adipose tissue, body fat percentage, and body mass index were assessed in a cross-sectional sample of 526 participants who attended health examinations in Yakumo, Japan. In addition, five circulating microRNAs (miR-20a, -21, -27a, -103a, and -320), which are involved in adipocyte proliferation and differentiation, were quantified using real-time polymerase chain reaction amplification. Results: We compared the circulating microRNA concentrations in a percentile greater than 75th (high) with below the value (low) of subcutaneous adipose tissue, visceral fat adipose tissue, body mass index, and per cent body fat. For visceral fat adipose tissue, significant decrease in miR-320 expression was observed in high group. Also, for body mass index, significant change of miR-20a, -27a, 103a, and 320 expression level was observed in high group. Multiple linear regression analysis demonstrated that circulating levels of some microRNA such as miR-27a were significantly associated with subcutaneous adipose tissue, visceral fat adipose tissue, and body mass index. Conclusions: Our findings support the need for further studies to determine whether such changes are consistent across different populations and whether the identified microRNAs may represent novel biomarkers to predict the susceptibility and progression of obesity-related disorders.

Original languageEnglish
Pages (from-to)437-445
Number of pages9
JournalAnnals of Clinical Biochemistry
Volume55
Issue number4
DOIs
Publication statusPublished - 01-07-2018

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Intra-Abdominal Fat
Subcutaneous Fat
MicroRNAs
Fats
Tissue
Population
Body Mass Index
Obesity
Adipose Tissue
Polymerase chain reaction
Adiposity
Biomarkers
Public health
Medical problems
Adipocytes
Linear regression
Regression analysis
Amplification
Real-Time Polymerase Chain Reaction
Linear Models

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

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title = "Association of subcutaneous and visceral fat with circulating microRNAs in a middle-aged Japanese population",
abstract = "Purpose: It has been demonstrated that circulating microRNA profiles are affected by physiological conditions. Several studies have demonstrated that microRNAs play important roles in the regulation of adiposity. However, few have investigated the relationship between circulating microRNAs and obesity, which has become a major public health problem worldwide. This study investigated the association between circulating microRNAs and obesity in a Japanese population. Methods: Obesity parameters, such as subcutaneous and visceral fat adipose tissue, body fat percentage, and body mass index were assessed in a cross-sectional sample of 526 participants who attended health examinations in Yakumo, Japan. In addition, five circulating microRNAs (miR-20a, -21, -27a, -103a, and -320), which are involved in adipocyte proliferation and differentiation, were quantified using real-time polymerase chain reaction amplification. Results: We compared the circulating microRNA concentrations in a percentile greater than 75th (high) with below the value (low) of subcutaneous adipose tissue, visceral fat adipose tissue, body mass index, and per cent body fat. For visceral fat adipose tissue, significant decrease in miR-320 expression was observed in high group. Also, for body mass index, significant change of miR-20a, -27a, 103a, and 320 expression level was observed in high group. Multiple linear regression analysis demonstrated that circulating levels of some microRNA such as miR-27a were significantly associated with subcutaneous adipose tissue, visceral fat adipose tissue, and body mass index. Conclusions: Our findings support the need for further studies to determine whether such changes are consistent across different populations and whether the identified microRNAs may represent novel biomarkers to predict the susceptibility and progression of obesity-related disorders.",
author = "Eiji Munetsuna and Hiroya Yamada and Yoshitaka Ando and Mirai Yamazaki and Yoshiki Tsuboi and Mari Kondo and Genki Mizuno and Hiroaki Ishikawa and Keiko Sugimoto and Keisuke Osakabe and Naohiro Ichino and Koji Ohashi and Nobuyuki Hamajima and Koji Suzuki",
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Association of subcutaneous and visceral fat with circulating microRNAs in a middle-aged Japanese population. / Munetsuna, Eiji; Yamada, Hiroya; Ando, Yoshitaka; Yamazaki, Mirai; Tsuboi, Yoshiki; Kondo, Mari; Mizuno, Genki; Ishikawa, Hiroaki; Sugimoto, Keiko; Osakabe, Keisuke; Ichino, Naohiro; Ohashi, Koji; Hamajima, Nobuyuki; Suzuki, Koji.

In: Annals of Clinical Biochemistry, Vol. 55, No. 4, 01.07.2018, p. 437-445.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Association of subcutaneous and visceral fat with circulating microRNAs in a middle-aged Japanese population

AU - Munetsuna, Eiji

AU - Yamada, Hiroya

AU - Ando, Yoshitaka

AU - Yamazaki, Mirai

AU - Tsuboi, Yoshiki

AU - Kondo, Mari

AU - Mizuno, Genki

AU - Ishikawa, Hiroaki

AU - Sugimoto, Keiko

AU - Osakabe, Keisuke

AU - Ichino, Naohiro

AU - Ohashi, Koji

AU - Hamajima, Nobuyuki

AU - Suzuki, Koji

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Purpose: It has been demonstrated that circulating microRNA profiles are affected by physiological conditions. Several studies have demonstrated that microRNAs play important roles in the regulation of adiposity. However, few have investigated the relationship between circulating microRNAs and obesity, which has become a major public health problem worldwide. This study investigated the association between circulating microRNAs and obesity in a Japanese population. Methods: Obesity parameters, such as subcutaneous and visceral fat adipose tissue, body fat percentage, and body mass index were assessed in a cross-sectional sample of 526 participants who attended health examinations in Yakumo, Japan. In addition, five circulating microRNAs (miR-20a, -21, -27a, -103a, and -320), which are involved in adipocyte proliferation and differentiation, were quantified using real-time polymerase chain reaction amplification. Results: We compared the circulating microRNA concentrations in a percentile greater than 75th (high) with below the value (low) of subcutaneous adipose tissue, visceral fat adipose tissue, body mass index, and per cent body fat. For visceral fat adipose tissue, significant decrease in miR-320 expression was observed in high group. Also, for body mass index, significant change of miR-20a, -27a, 103a, and 320 expression level was observed in high group. Multiple linear regression analysis demonstrated that circulating levels of some microRNA such as miR-27a were significantly associated with subcutaneous adipose tissue, visceral fat adipose tissue, and body mass index. Conclusions: Our findings support the need for further studies to determine whether such changes are consistent across different populations and whether the identified microRNAs may represent novel biomarkers to predict the susceptibility and progression of obesity-related disorders.

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