Associations between polymorphisms of interleukin-6 and related cytokine genes and serum liver damage markers: A cross-sectional study in the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study

for the J-MICC Study Group

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Abstract

Cytokines, including interleukin-6 (IL-6), play an important role in the liver. The aim of this study was to investigate associations between common polymorphisms in potential functional promoters of cytokine genes and liver damage markers among enrollees of a large Japanese cohort study. Subjects included 3257 Japanese individuals (1608 men and 1649 women, aged 35-69. years). Six single nucleotide polymorphisms (SNPs) in the promoter regions of five cytokine genes, IL1B (T-31C), IL6 (C-634G), IL8 (T-251A), IL10 (T-819C), tumor necrosis factor-A (TNFA) (T-1031C), and TNFA (C-857T), were genotyped by polymerase chain reaction. Information regarding alcohol intake, smoking habits, height, and weight was collected by a self-administered questionnaire. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured during a routine health check-up. Of the six SNPs genotyped, an IL6 polymorphism (rs1800796, C-634G) was most strongly associated with a liver damage marker, AST. Mean serum AST was significantly different among the three genotypes (mean. ±. SD, 22.7. ±. 7.3. IU/L for CC, 22.8. ±. 7.7. IU/L for CG, and 24.3. ±. 8.6. IU/L for GG, p= 0.011 by analysis of variance). The differences remained significant after adjustment for potential confounders by general linear models. The variations in mean serum AST and ALT levels were marked especially among men. Thus, the functional polymorphism IL6 C-634G may affect serum AST and ALT levels, possibly through different IL-6 production.

Original languageEnglish
Pages (from-to)158-162
Number of pages5
JournalGene
Volume557
Issue number2
DOIs
Publication statusPublished - 25-02-2015

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Aspartate Aminotransferases
Interleukin-6
Japan
Cohort Studies
Cross-Sectional Studies
Cytokines
Alanine Transaminase
Liver
Serum
Genes
Single Nucleotide Polymorphism
Lymphotoxin-beta
Interleukin-8
Genetic Promoter Regions
Interleukin-10
Habits
Linear Models
Analysis of Variance
Tumor Necrosis Factor-alpha
Smoking

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

@article{bc8fdd81717545f299c6498203277319,
title = "Associations between polymorphisms of interleukin-6 and related cytokine genes and serum liver damage markers: A cross-sectional study in the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study",
abstract = "Cytokines, including interleukin-6 (IL-6), play an important role in the liver. The aim of this study was to investigate associations between common polymorphisms in potential functional promoters of cytokine genes and liver damage markers among enrollees of a large Japanese cohort study. Subjects included 3257 Japanese individuals (1608 men and 1649 women, aged 35-69. years). Six single nucleotide polymorphisms (SNPs) in the promoter regions of five cytokine genes, IL1B (T-31C), IL6 (C-634G), IL8 (T-251A), IL10 (T-819C), tumor necrosis factor-A (TNFA) (T-1031C), and TNFA (C-857T), were genotyped by polymerase chain reaction. Information regarding alcohol intake, smoking habits, height, and weight was collected by a self-administered questionnaire. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured during a routine health check-up. Of the six SNPs genotyped, an IL6 polymorphism (rs1800796, C-634G) was most strongly associated with a liver damage marker, AST. Mean serum AST was significantly different among the three genotypes (mean. ±. SD, 22.7. ±. 7.3. IU/L for CC, 22.8. ±. 7.7. IU/L for CG, and 24.3. ±. 8.6. IU/L for GG, p= 0.011 by analysis of variance). The differences remained significant after adjustment for potential confounders by general linear models. The variations in mean serum AST and ALT levels were marked especially among men. Thus, the functional polymorphism IL6 C-634G may affect serum AST and ALT levels, possibly through different IL-6 production.",
author = "{for the J-MICC Study Group} and Yuka Sugimoto and Kenji Wakai and Hiroko Nakagawa and Shino Suma and Tae Sasakabe and Tatsuhiko Sakamoto and Naoyuki Takashima and Sadao Suzuki and Shin Ogawa and Keizo Ohnaka and Nagato Kuriyama and Kokichi Arisawa and Haruo Mikami and Michiaki Kubo and Michiaki Kubo and Nobuyuki Hamajima and Hideo Tanaka",
year = "2015",
month = "2",
day = "25",
doi = "10.1016/j.gene.2014.12.025",
language = "English",
volume = "557",
pages = "158--162",
journal = "Gene",
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T1 - Associations between polymorphisms of interleukin-6 and related cytokine genes and serum liver damage markers

T2 - A cross-sectional study in the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study

AU - for the J-MICC Study Group

AU - Sugimoto, Yuka

AU - Wakai, Kenji

AU - Nakagawa, Hiroko

AU - Suma, Shino

AU - Sasakabe, Tae

AU - Sakamoto, Tatsuhiko

AU - Takashima, Naoyuki

AU - Suzuki, Sadao

AU - Ogawa, Shin

AU - Ohnaka, Keizo

AU - Kuriyama, Nagato

AU - Arisawa, Kokichi

AU - Mikami, Haruo

AU - Kubo, Michiaki

AU - Kubo, Michiaki

AU - Hamajima, Nobuyuki

AU - Tanaka, Hideo

PY - 2015/2/25

Y1 - 2015/2/25

N2 - Cytokines, including interleukin-6 (IL-6), play an important role in the liver. The aim of this study was to investigate associations between common polymorphisms in potential functional promoters of cytokine genes and liver damage markers among enrollees of a large Japanese cohort study. Subjects included 3257 Japanese individuals (1608 men and 1649 women, aged 35-69. years). Six single nucleotide polymorphisms (SNPs) in the promoter regions of five cytokine genes, IL1B (T-31C), IL6 (C-634G), IL8 (T-251A), IL10 (T-819C), tumor necrosis factor-A (TNFA) (T-1031C), and TNFA (C-857T), were genotyped by polymerase chain reaction. Information regarding alcohol intake, smoking habits, height, and weight was collected by a self-administered questionnaire. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured during a routine health check-up. Of the six SNPs genotyped, an IL6 polymorphism (rs1800796, C-634G) was most strongly associated with a liver damage marker, AST. Mean serum AST was significantly different among the three genotypes (mean. ±. SD, 22.7. ±. 7.3. IU/L for CC, 22.8. ±. 7.7. IU/L for CG, and 24.3. ±. 8.6. IU/L for GG, p= 0.011 by analysis of variance). The differences remained significant after adjustment for potential confounders by general linear models. The variations in mean serum AST and ALT levels were marked especially among men. Thus, the functional polymorphism IL6 C-634G may affect serum AST and ALT levels, possibly through different IL-6 production.

AB - Cytokines, including interleukin-6 (IL-6), play an important role in the liver. The aim of this study was to investigate associations between common polymorphisms in potential functional promoters of cytokine genes and liver damage markers among enrollees of a large Japanese cohort study. Subjects included 3257 Japanese individuals (1608 men and 1649 women, aged 35-69. years). Six single nucleotide polymorphisms (SNPs) in the promoter regions of five cytokine genes, IL1B (T-31C), IL6 (C-634G), IL8 (T-251A), IL10 (T-819C), tumor necrosis factor-A (TNFA) (T-1031C), and TNFA (C-857T), were genotyped by polymerase chain reaction. Information regarding alcohol intake, smoking habits, height, and weight was collected by a self-administered questionnaire. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured during a routine health check-up. Of the six SNPs genotyped, an IL6 polymorphism (rs1800796, C-634G) was most strongly associated with a liver damage marker, AST. Mean serum AST was significantly different among the three genotypes (mean. ±. SD, 22.7. ±. 7.3. IU/L for CC, 22.8. ±. 7.7. IU/L for CG, and 24.3. ±. 8.6. IU/L for GG, p= 0.011 by analysis of variance). The differences remained significant after adjustment for potential confounders by general linear models. The variations in mean serum AST and ALT levels were marked especially among men. Thus, the functional polymorphism IL6 C-634G may affect serum AST and ALT levels, possibly through different IL-6 production.

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