Associations of serum microRNA-20a, -27a, and -103a with cognitive function in a Japanese population: The Yakumo study

Mari Kondo, Hiroya Yamada, Eiji Munetsuna, Mirai Yamazaki, Takeshi Hatta, Akihiko Iwahara, Koji Ohashi, Hiroaki Ishikawa, Yoshiki Tsuboi, Takashi Inoue, Ryosuke Fujii, Koji Suzuki

Research output: Contribution to journalArticle

Abstract

Objectives: MicroRNAs (miRNAs) dysregulate gene expression by binding to target messenger RNAs, and play an important role in the pathogenesis of various diseases, including cancers, cardiovascular diseases and diabetes. Circulating miRNAs have increasingly been recognized as biomarkers for detecting and diagnosing those diseases. Few studies have investigated the association of circulating miRNA with the early stages of cognitive impairment, such as mild cognitive impairment, in the general population. The purpose of this study was to examine the association between cognitive function and several serum miRNAs levels related to amyloid precursor protein (APP) proteolysis in a Japanese general population who had never been diagnosed with dementia. Methods: We conducted a cross-sectional study of 337 Japanese subjects (144 men, 193 women) who attended a health examination. The short form of the Mini-Mental State Examination (SMMSE) was used to assess cognitive function. Serum levels of 6 miRNAs (let-7d, miR-17, miR-20a, miR-27a, miR-34a, miR-103a) were measured by quantitative real-time polymerase chain reaction. Results: Multivariable-adjusted odds ratios (ORs) for lower SMMSE score (SMMSE score < 28) were significantly increased in the lowest tertile of serum miR-20a (OR, 2.08; 95% confidence interval (CI), 1.09–4.04) and miR-103a (OR, 1.91; 95%CI, 1.00–3.69) compared to the highest tertile. Moreover, serum levels of miR-20a, -27a, and -103a were linearly and positively associated with SMMSE scores after adjustment for confounding factors. Conclusion: Low serum levels of miR-20a, -27a, and -103a are independently associated with cognitive impairment.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalArchives of Gerontology and Geriatrics
Volume82
DOIs
Publication statusPublished - 01-05-2019

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MicroRNAs
Cognition
examination
Serum
Population
Odds Ratio
Disease
confidence
Confidence Intervals
Amyloid beta-Protein Precursor
dementia
cross-sectional study
Proteolysis
chronic illness
Dementia
Real-Time Polymerase Chain Reaction
cancer
Cardiovascular Diseases
Cross-Sectional Studies
Biomarkers

All Science Journal Classification (ASJC) codes

  • Health(social science)
  • Ageing
  • Gerontology
  • Geriatrics and Gerontology

Cite this

@article{bd261d584e264d1187322be4c34dc535,
title = "Associations of serum microRNA-20a, -27a, and -103a with cognitive function in a Japanese population: The Yakumo study",
abstract = "Objectives: MicroRNAs (miRNAs) dysregulate gene expression by binding to target messenger RNAs, and play an important role in the pathogenesis of various diseases, including cancers, cardiovascular diseases and diabetes. Circulating miRNAs have increasingly been recognized as biomarkers for detecting and diagnosing those diseases. Few studies have investigated the association of circulating miRNA with the early stages of cognitive impairment, such as mild cognitive impairment, in the general population. The purpose of this study was to examine the association between cognitive function and several serum miRNAs levels related to amyloid precursor protein (APP) proteolysis in a Japanese general population who had never been diagnosed with dementia. Methods: We conducted a cross-sectional study of 337 Japanese subjects (144 men, 193 women) who attended a health examination. The short form of the Mini-Mental State Examination (SMMSE) was used to assess cognitive function. Serum levels of 6 miRNAs (let-7d, miR-17, miR-20a, miR-27a, miR-34a, miR-103a) were measured by quantitative real-time polymerase chain reaction. Results: Multivariable-adjusted odds ratios (ORs) for lower SMMSE score (SMMSE score < 28) were significantly increased in the lowest tertile of serum miR-20a (OR, 2.08; 95{\%} confidence interval (CI), 1.09–4.04) and miR-103a (OR, 1.91; 95{\%}CI, 1.00–3.69) compared to the highest tertile. Moreover, serum levels of miR-20a, -27a, and -103a were linearly and positively associated with SMMSE scores after adjustment for confounding factors. Conclusion: Low serum levels of miR-20a, -27a, and -103a are independently associated with cognitive impairment.",
author = "Mari Kondo and Hiroya Yamada and Eiji Munetsuna and Mirai Yamazaki and Takeshi Hatta and Akihiko Iwahara and Koji Ohashi and Hiroaki Ishikawa and Yoshiki Tsuboi and Takashi Inoue and Ryosuke Fujii and Koji Suzuki",
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doi = "10.1016/j.archger.2019.01.007",
language = "English",
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pages = "155--160",
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Associations of serum microRNA-20a, -27a, and -103a with cognitive function in a Japanese population : The Yakumo study. / Kondo, Mari; Yamada, Hiroya; Munetsuna, Eiji; Yamazaki, Mirai; Hatta, Takeshi; Iwahara, Akihiko; Ohashi, Koji; Ishikawa, Hiroaki; Tsuboi, Yoshiki; Inoue, Takashi; Fujii, Ryosuke; Suzuki, Koji.

In: Archives of Gerontology and Geriatrics, Vol. 82, 01.05.2019, p. 155-160.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Associations of serum microRNA-20a, -27a, and -103a with cognitive function in a Japanese population

T2 - The Yakumo study

AU - Kondo, Mari

AU - Yamada, Hiroya

AU - Munetsuna, Eiji

AU - Yamazaki, Mirai

AU - Hatta, Takeshi

AU - Iwahara, Akihiko

AU - Ohashi, Koji

AU - Ishikawa, Hiroaki

AU - Tsuboi, Yoshiki

AU - Inoue, Takashi

AU - Fujii, Ryosuke

AU - Suzuki, Koji

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Objectives: MicroRNAs (miRNAs) dysregulate gene expression by binding to target messenger RNAs, and play an important role in the pathogenesis of various diseases, including cancers, cardiovascular diseases and diabetes. Circulating miRNAs have increasingly been recognized as biomarkers for detecting and diagnosing those diseases. Few studies have investigated the association of circulating miRNA with the early stages of cognitive impairment, such as mild cognitive impairment, in the general population. The purpose of this study was to examine the association between cognitive function and several serum miRNAs levels related to amyloid precursor protein (APP) proteolysis in a Japanese general population who had never been diagnosed with dementia. Methods: We conducted a cross-sectional study of 337 Japanese subjects (144 men, 193 women) who attended a health examination. The short form of the Mini-Mental State Examination (SMMSE) was used to assess cognitive function. Serum levels of 6 miRNAs (let-7d, miR-17, miR-20a, miR-27a, miR-34a, miR-103a) were measured by quantitative real-time polymerase chain reaction. Results: Multivariable-adjusted odds ratios (ORs) for lower SMMSE score (SMMSE score < 28) were significantly increased in the lowest tertile of serum miR-20a (OR, 2.08; 95% confidence interval (CI), 1.09–4.04) and miR-103a (OR, 1.91; 95%CI, 1.00–3.69) compared to the highest tertile. Moreover, serum levels of miR-20a, -27a, and -103a were linearly and positively associated with SMMSE scores after adjustment for confounding factors. Conclusion: Low serum levels of miR-20a, -27a, and -103a are independently associated with cognitive impairment.

AB - Objectives: MicroRNAs (miRNAs) dysregulate gene expression by binding to target messenger RNAs, and play an important role in the pathogenesis of various diseases, including cancers, cardiovascular diseases and diabetes. Circulating miRNAs have increasingly been recognized as biomarkers for detecting and diagnosing those diseases. Few studies have investigated the association of circulating miRNA with the early stages of cognitive impairment, such as mild cognitive impairment, in the general population. The purpose of this study was to examine the association between cognitive function and several serum miRNAs levels related to amyloid precursor protein (APP) proteolysis in a Japanese general population who had never been diagnosed with dementia. Methods: We conducted a cross-sectional study of 337 Japanese subjects (144 men, 193 women) who attended a health examination. The short form of the Mini-Mental State Examination (SMMSE) was used to assess cognitive function. Serum levels of 6 miRNAs (let-7d, miR-17, miR-20a, miR-27a, miR-34a, miR-103a) were measured by quantitative real-time polymerase chain reaction. Results: Multivariable-adjusted odds ratios (ORs) for lower SMMSE score (SMMSE score < 28) were significantly increased in the lowest tertile of serum miR-20a (OR, 2.08; 95% confidence interval (CI), 1.09–4.04) and miR-103a (OR, 1.91; 95%CI, 1.00–3.69) compared to the highest tertile. Moreover, serum levels of miR-20a, -27a, and -103a were linearly and positively associated with SMMSE scores after adjustment for confounding factors. Conclusion: Low serum levels of miR-20a, -27a, and -103a are independently associated with cognitive impairment.

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DO - 10.1016/j.archger.2019.01.007

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