Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG

Yuki Arimura; Yusuke Minato; Takayuki Wada; Masaaki Nakayama; Ayako Ryumon; Nao Hirata; Chie Nakajima; Yasuhiko Suzuki; Manabu Ato; Kazuo Kobayashi; Naoko Ohara; Seiji Iida; Naoya Ohara

doi:10.1111/1348-0421.12944

Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG

Yuki Arimura
, Yusuke Minato
, Takayuki Wada
, Masaaki Nakayama
, Ayako Ryumon
, Nao Hirata
, Chie Nakajima
, Yasuhiko Suzuki
, Manabu Ato
, Kazuo Kobayashi
, Naoko Ohara
, Seiji Iida
, Naoya Ohara

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.

Original language	English
Pages (from-to)	10-14
Number of pages	5
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	66
Issue number	1
DOIs	https://doi.org/10.1111/1348-0421.12944
Publication status	Published - 01-2022
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Virology

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10.1111/1348-0421.12944

Cite this

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title = "Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG",

abstract = "Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.",

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TY - JOUR

T1 - Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG

AU - Arimura, Yuki

AU - Minato, Yusuke

AU - Wada, Takayuki

AU - Nakayama, Masaaki

AU - Ryumon, Ayako

AU - Hirata, Nao

AU - Nakajima, Chie

AU - Suzuki, Yasuhiko

AU - Ato, Manabu

AU - Kobayashi, Kazuo

AU - Ohara, Naoko

AU - Iida, Seiji

AU - Ohara, Naoya

PY - 2022/1

Y1 - 2022/1

N2 - Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.

AB - Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.

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DO - 10.1111/1348-0421.12944

M3 - Article

C2 - 34546594

AN - SCOPUS:85117500937

SN - 0385-5600

VL - 66

SP - 10

EP - 14

JO - MICROBIOLOGY and IMMUNOLOGY

JF - MICROBIOLOGY and IMMUNOLOGY

IS - 1

ER -

Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG

Abstract

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