Attenuated c-fos mRNA induction after middle cerebral artery occlusion in CREB knockout mice does not modulate focal ischemic injury

Ryuji Hata, Peter Gass, Günter Mies, Christoph Wiessner, Konstantin Alexander Hossmann

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

To elucidate the mechanism of ischemia-induced signal transduction in vivo, we investigated the effect of the targeted disruption of the α and Δ isoforms of the cAMP-responsive element-binding protein (CREB) on c-fos and heat-shock protein (hsp) 72 gene induction. Permanent focal ischemia was induced by occlusion of the middle cerebral artery of the CREB mutant mice (CREB(-/-), n = 5) and the wild-type mice (n = 6). Three hours after onset of ischemia, the neurologic score was assessed and pictorial measurements of ATP and cerebral protein synthesis (CPS) were carried out to differentiate between the ischemic core (where ATP is depleted), the ischemic penumbra (where ATP is preserved but CPS is inhibited), and the intact tissue (where both ATP and CPS are preserved). There were no significant differences in neurologic score or in ATP, pH, and CPS between the two groups, suggesting that the sensitivity of both strains to ischemia is the same. Targeted disruption of the CREB gene significantly attenuated c-fos gene induction in the periischemic ipsilateral hemisphere but had no effect on either c-fos or hsp72 mRNA expression in the penumbra. The observations demonstrate that CREB expression, despite its differential effect on c-fos, does not modulate acute focal ischemic injury.

Original languageEnglish
Pages (from-to)1325-1335
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume18
Issue number12
DOIs
Publication statusPublished - 01-01-1998

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Middle Cerebral Artery Infarction
Knockout Mice
Carrier Proteins
Adenosine Triphosphate
Ischemia
Messenger RNA
Wounds and Injuries
Nervous System
Proteins
HSP72 Heat-Shock Proteins
fos Genes
Genes
Signal Transduction
Protein Isoforms

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Hata, Ryuji ; Gass, Peter ; Mies, Günter ; Wiessner, Christoph ; Hossmann, Konstantin Alexander. / Attenuated c-fos mRNA induction after middle cerebral artery occlusion in CREB knockout mice does not modulate focal ischemic injury. In: Journal of Cerebral Blood Flow and Metabolism. 1998 ; Vol. 18, No. 12. pp. 1325-1335.
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Attenuated c-fos mRNA induction after middle cerebral artery occlusion in CREB knockout mice does not modulate focal ischemic injury. / Hata, Ryuji; Gass, Peter; Mies, Günter; Wiessner, Christoph; Hossmann, Konstantin Alexander.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 18, No. 12, 01.01.1998, p. 1325-1335.

Research output: Contribution to journalArticle

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