Attenuation of fibroblast growth factor signaling by poly-N- acetyllactosamine type glycans

Kazuhiro Sugihara, Toshiaki K. Shibata, Kayoko Takata, Takako Kimura, Naohiro Kanayama, Roy Williams, Shingo Hatakeyama, Tomoya O. Akama, Chu Wei Kuo, Kay Hooi Khoo, Michiko N. Fukuda

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5 Citations (Scopus)

Abstract

Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glycans (polyLacs), enhanced motility. Mass spectrometry analysis revealed that sperm-associated polyLacs are heavily fucosylated, consistent with Lewis Y antigen. Immunohistochemistry of epididymis using an anti-Lewis Y antibody before and after EBG treatment suggested that polyLacs carrying the Lewis Y epitope are synthesized in epididymal epithelia and secreted to seminal fluid. EBG-treated sperm elevated cAMP levels and calcium influx, indicating activation of fibroblast growth factor signaling. Seminal fluid polyLacs bound to FGFs in vitro, and impaired FGF-mediated signaling in HEK293T cells.

Original languageEnglish
Pages (from-to)3195-3201
Number of pages7
JournalFEBS Letters
Volume587
Issue number19
DOIs
Publication statusPublished - 01-10-2013

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Sugihara, K., Shibata, T. K., Takata, K., Kimura, T., Kanayama, N., Williams, R., Hatakeyama, S., Akama, T. O., Kuo, C. W., Khoo, K. H., & Fukuda, M. N. (2013). Attenuation of fibroblast growth factor signaling by poly-N- acetyllactosamine type glycans. FEBS Letters, 587(19), 3195-3201. https://doi.org/10.1016/j.febslet.2013.07.056