TY - JOUR
T1 - Augmenting Effect of Antibiotics on Endotoxin Activity May Cause A Safety Problem
AU - Yamamoto, Akihiko
AU - Sakai, Takeo
AU - Ochiai, Masaki
AU - Kamachi, Kazunari
AU - Kataoka, Michiyo
AU - Toyoizumi, Hiromi
AU - Arakawa, Yoshichika
AU - Horiuchi, Yoshinobu
PY - 2004
Y1 - 2004
N2 - Enhancing/interfering effect of antibiotics on endotoxin was evaluated using the endotoxin test and the cell line assay in 28SC cells that has a responsiveness consistent with that of human peripheral blood. When a total of 21 products of seven different kinds of antibiotics were tested, none showed any significant effect on the endotoxin test at its therapeutic dose. However, aminoglycosides showed a significant augmenting effect on IL-6 induction of endotoxin in 28SC cells. Detailed examination of the augmenting effect was made on spectinomycin in the in vitro cell line assay and also in the lethal endotoxin challenge assay in D-galactosamine-treated mice. Spectinomycin also enhanced the endotoxin lethality in D-galactosamine-treated mice. A kinetic analysis in endotoxin-sensitized 28SC cells revealed that the augmentation takes place as quickly as 10 min after spectinomycin treatment. Accordingly, a special caution concerning the augmenting effect was assumed necessary for the safety control of antibiotic products as well as for selecting antibiotics for the therapeutic use.
AB - Enhancing/interfering effect of antibiotics on endotoxin was evaluated using the endotoxin test and the cell line assay in 28SC cells that has a responsiveness consistent with that of human peripheral blood. When a total of 21 products of seven different kinds of antibiotics were tested, none showed any significant effect on the endotoxin test at its therapeutic dose. However, aminoglycosides showed a significant augmenting effect on IL-6 induction of endotoxin in 28SC cells. Detailed examination of the augmenting effect was made on spectinomycin in the in vitro cell line assay and also in the lethal endotoxin challenge assay in D-galactosamine-treated mice. Spectinomycin also enhanced the endotoxin lethality in D-galactosamine-treated mice. A kinetic analysis in endotoxin-sensitized 28SC cells revealed that the augmentation takes place as quickly as 10 min after spectinomycin treatment. Accordingly, a special caution concerning the augmenting effect was assumed necessary for the safety control of antibiotic products as well as for selecting antibiotics for the therapeutic use.
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U2 - 10.1111/j.1348-0421.2004.tb03494.x
DO - 10.1111/j.1348-0421.2004.tb03494.x
M3 - Article
C2 - 14978334
AN - SCOPUS:1342283147
SN - 0385-5600
VL - 48
SP - 97
EP - 102
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 2
ER -