Aurora-B associated protein phosphatases as negative regulators of kinase activation

Keiichi Sugiyama, Kazumitsu Sugiura, Tomohiro Hara, Kenji Sugimoto, Hiroshi Shima, Kei Honda, Koichi Furukawa, Shunichi Yamashita, Takeshi Urano

Research output: Contribution to journalArticle

Abstract

The human serine/threonine kinase Aurora-B is structurally related to the protein kinase Ipl1p from S cerevisiae and aurora from Drosophila melanogaster, which are key regulators of mitosis. The present study shows that human Aurora-B is activated by okadaic acid and forms complexes with the protein serine/threonine phosphatase type 1 (PP1) or PP2A, but not with PP5. These data identified Aurora-B associated protein phosphatases as negative regulators of kinase activation. We then used a series of substrates based on a histone H3 phosphorylation site (residues 5-15) to determine the substrate specificity of human Aurora-B. We found that this enzyme is an arginine-directed kinase that can phosphorylate histone H3 at serines 10 and 28 in vitro, suggesting that human Aurora-B is a mitotic histone H3 kinase.

Original languageEnglish
Pages (from-to)3103-3111
Number of pages9
JournalOncogene
Volume21
Issue number20
DOIs
Publication statusPublished - 09-05-2002
Externally publishedYes

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Phosphoprotein Phosphatases
Phosphotransferases
Histones
Arginine Kinase
Protamine Kinase
Protein Phosphatase 1
Okadaic Acid
Protein-Serine-Threonine Kinases
Substrate Specificity
Drosophila melanogaster
Mitosis
Serine
Phosphorylation
Enzymes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Sugiyama, K., Sugiura, K., Hara, T., Sugimoto, K., Shima, H., Honda, K., ... Urano, T. (2002). Aurora-B associated protein phosphatases as negative regulators of kinase activation. Oncogene, 21(20), 3103-3111. https://doi.org/10.1038/sj/onc/1205432
Sugiyama, Keiichi ; Sugiura, Kazumitsu ; Hara, Tomohiro ; Sugimoto, Kenji ; Shima, Hiroshi ; Honda, Kei ; Furukawa, Koichi ; Yamashita, Shunichi ; Urano, Takeshi. / Aurora-B associated protein phosphatases as negative regulators of kinase activation. In: Oncogene. 2002 ; Vol. 21, No. 20. pp. 3103-3111.
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Sugiyama, K, Sugiura, K, Hara, T, Sugimoto, K, Shima, H, Honda, K, Furukawa, K, Yamashita, S & Urano, T 2002, 'Aurora-B associated protein phosphatases as negative regulators of kinase activation', Oncogene, vol. 21, no. 20, pp. 3103-3111. https://doi.org/10.1038/sj/onc/1205432

Aurora-B associated protein phosphatases as negative regulators of kinase activation. / Sugiyama, Keiichi; Sugiura, Kazumitsu; Hara, Tomohiro; Sugimoto, Kenji; Shima, Hiroshi; Honda, Kei; Furukawa, Koichi; Yamashita, Shunichi; Urano, Takeshi.

In: Oncogene, Vol. 21, No. 20, 09.05.2002, p. 3103-3111.

Research output: Contribution to journalArticle

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