TY - JOUR
T1 - Aurora-B associated protein phosphatases as negative regulators of kinase activation
AU - Sugiyama, Keiichi
AU - Sugiura, Kazumitsu
AU - Hara, Tomohiro
AU - Sugimoto, Kenji
AU - Shima, Hiroshi
AU - Honda, Kei
AU - Furukawa, Koichi
AU - Yamashita, Shunichi
AU - Urano, Takeshi
N1 - Funding Information:
We thank Dr Tadashi Yamamoto (University of Tokyo, Tokyo) for pME-Gex4T-1 and Ms Tomoko Tsurutome for excellent technical assistance. This work was supported by grants-in-aid for Scientific Research and Core of Excellence from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2002
Y1 - 2002
N2 - The human serine/threonine kinase Aurora-B is structurally related to the protein kinase Ipl1p from S cerevisiae and aurora from Drosophila melanogaster, which are key regulators of mitosis. The present study shows that human Aurora-B is activated by okadaic acid and forms complexes with the protein serine/threonine phosphatase type 1 (PP1) or PP2A, but not with PP5. These data identified Aurora-B associated protein phosphatases as negative regulators of kinase activation. We then used a series of substrates based on a histone H3 phosphorylation site (residues 5-15) to determine the substrate specificity of human Aurora-B. We found that this enzyme is an arginine-directed kinase that can phosphorylate histone H3 at serines 10 and 28 in vitro, suggesting that human Aurora-B is a mitotic histone H3 kinase.
AB - The human serine/threonine kinase Aurora-B is structurally related to the protein kinase Ipl1p from S cerevisiae and aurora from Drosophila melanogaster, which are key regulators of mitosis. The present study shows that human Aurora-B is activated by okadaic acid and forms complexes with the protein serine/threonine phosphatase type 1 (PP1) or PP2A, but not with PP5. These data identified Aurora-B associated protein phosphatases as negative regulators of kinase activation. We then used a series of substrates based on a histone H3 phosphorylation site (residues 5-15) to determine the substrate specificity of human Aurora-B. We found that this enzyme is an arginine-directed kinase that can phosphorylate histone H3 at serines 10 and 28 in vitro, suggesting that human Aurora-B is a mitotic histone H3 kinase.
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U2 - 10.1038/sj.onc.1205432
DO - 10.1038/sj.onc.1205432
M3 - Article
C2 - 12082625
AN - SCOPUS:85047696924
SN - 0950-9232
VL - 21
SP - 3103
EP - 3111
JO - Oncogene
JF - Oncogene
IS - 20
ER -
