Autoantibodies to DFS70/LEDGF are increased in alopecia areata patients

Miyako Okamoto, Yasushi Ogawa, Akihiro Watanabe, Kazumitsu Sugiura, Yutaka Shimomura, Noriaki Aoki, Tetsuro Nagasaka, Yasushi Tomita, Yoshinao Muro

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Alopecia areata (AA) has been suspected to be an autoimmune disease, although there is no distinct evidence, we investigated the relationship between AA and autoantibodies against dense fine speckles 70 kDa (DFS70) in 111 patients with alopecia and 105 healthy controls. The sera from 59 out of 111 (53%) Japanese alopecia patients were positive for anti-nuclear antibody (ANA), as compared to the sera of 16 out of 105 (15%) healthy controls (p < 0.001). Twenty percent (22/111) of the alopecia patients were shown to be positive for the prevalence of anti-DFS70 antibodies, as compared to 8% (8/105) of the healthy controls (p < 0.01). IgG subclass analysis by ELISA showed that IgG1 and IgG2-anti-DFS70 antibodies were dominant in alopecia patients. The DFS70 gene expression in the hair structures was clearly detected in both those with and those without the anti-DFS70 antibody by RT-PCR. Immunohistochemical techniques showed that the DFS70 was localized predominantly in the outer root sheath (ORS) cells. The elevated anti-DFS70 antibodies in alopecia patients and the localization of the DFS70 in the ORS suggest that autoantibodies against the DFS70 are related to the etiology in a certain population of AA.

Original languageEnglish
Pages (from-to)257-266
Number of pages10
JournalJournal of Autoimmunity
Volume23
Issue number3
DOIs
Publication statusPublished - 01-01-2004

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Alopecia Areata
Alopecia
Autoantibodies
Antibodies
Immunoglobulin G
Serum
Hair
Autoimmune Diseases
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
lens epithelium-derived growth factor
Gene Expression
Polymerase Chain Reaction
Population

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Okamoto, M., Ogawa, Y., Watanabe, A., Sugiura, K., Shimomura, Y., Aoki, N., ... Muro, Y. (2004). Autoantibodies to DFS70/LEDGF are increased in alopecia areata patients. Journal of Autoimmunity, 23(3), 257-266. https://doi.org/10.1016/j.jaut.2004.07.004
Okamoto, Miyako ; Ogawa, Yasushi ; Watanabe, Akihiro ; Sugiura, Kazumitsu ; Shimomura, Yutaka ; Aoki, Noriaki ; Nagasaka, Tetsuro ; Tomita, Yasushi ; Muro, Yoshinao. / Autoantibodies to DFS70/LEDGF are increased in alopecia areata patients. In: Journal of Autoimmunity. 2004 ; Vol. 23, No. 3. pp. 257-266.
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abstract = "Alopecia areata (AA) has been suspected to be an autoimmune disease, although there is no distinct evidence, we investigated the relationship between AA and autoantibodies against dense fine speckles 70 kDa (DFS70) in 111 patients with alopecia and 105 healthy controls. The sera from 59 out of 111 (53{\%}) Japanese alopecia patients were positive for anti-nuclear antibody (ANA), as compared to the sera of 16 out of 105 (15{\%}) healthy controls (p < 0.001). Twenty percent (22/111) of the alopecia patients were shown to be positive for the prevalence of anti-DFS70 antibodies, as compared to 8{\%} (8/105) of the healthy controls (p < 0.01). IgG subclass analysis by ELISA showed that IgG1 and IgG2-anti-DFS70 antibodies were dominant in alopecia patients. The DFS70 gene expression in the hair structures was clearly detected in both those with and those without the anti-DFS70 antibody by RT-PCR. Immunohistochemical techniques showed that the DFS70 was localized predominantly in the outer root sheath (ORS) cells. The elevated anti-DFS70 antibodies in alopecia patients and the localization of the DFS70 in the ORS suggest that autoantibodies against the DFS70 are related to the etiology in a certain population of AA.",
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Okamoto, M, Ogawa, Y, Watanabe, A, Sugiura, K, Shimomura, Y, Aoki, N, Nagasaka, T, Tomita, Y & Muro, Y 2004, 'Autoantibodies to DFS70/LEDGF are increased in alopecia areata patients', Journal of Autoimmunity, vol. 23, no. 3, pp. 257-266. https://doi.org/10.1016/j.jaut.2004.07.004

Autoantibodies to DFS70/LEDGF are increased in alopecia areata patients. / Okamoto, Miyako; Ogawa, Yasushi; Watanabe, Akihiro; Sugiura, Kazumitsu; Shimomura, Yutaka; Aoki, Noriaki; Nagasaka, Tetsuro; Tomita, Yasushi; Muro, Yoshinao.

In: Journal of Autoimmunity, Vol. 23, No. 3, 01.01.2004, p. 257-266.

Research output: Contribution to journalArticle

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T1 - Autoantibodies to DFS70/LEDGF are increased in alopecia areata patients

AU - Okamoto, Miyako

AU - Ogawa, Yasushi

AU - Watanabe, Akihiro

AU - Sugiura, Kazumitsu

AU - Shimomura, Yutaka

AU - Aoki, Noriaki

AU - Nagasaka, Tetsuro

AU - Tomita, Yasushi

AU - Muro, Yoshinao

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AB - Alopecia areata (AA) has been suspected to be an autoimmune disease, although there is no distinct evidence, we investigated the relationship between AA and autoantibodies against dense fine speckles 70 kDa (DFS70) in 111 patients with alopecia and 105 healthy controls. The sera from 59 out of 111 (53%) Japanese alopecia patients were positive for anti-nuclear antibody (ANA), as compared to the sera of 16 out of 105 (15%) healthy controls (p < 0.001). Twenty percent (22/111) of the alopecia patients were shown to be positive for the prevalence of anti-DFS70 antibodies, as compared to 8% (8/105) of the healthy controls (p < 0.01). IgG subclass analysis by ELISA showed that IgG1 and IgG2-anti-DFS70 antibodies were dominant in alopecia patients. The DFS70 gene expression in the hair structures was clearly detected in both those with and those without the anti-DFS70 antibody by RT-PCR. Immunohistochemical techniques showed that the DFS70 was localized predominantly in the outer root sheath (ORS) cells. The elevated anti-DFS70 antibodies in alopecia patients and the localization of the DFS70 in the ORS suggest that autoantibodies against the DFS70 are related to the etiology in a certain population of AA.

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