TY - JOUR
T1 - Autoantibody response against a novel testicular antigen protein highly expressed in testis (PHET) in SSc patients
AU - Yasuoka, Hidekata
AU - Kuwana, Masataka
N1 - Funding Information:
This work was supported by Keio University Medical Science Fund, a grant from the Ministry of Health, Labor and Welfare, and a grant from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2007/3
Y1 - 2007/3
N2 - Systemic sclerosis (SSc) is characterized by excessive fibrosis and autoantibody production. However, the pathogenesis of SSc is still under investigation. We have demonstrated that a novel testicular antigen, protein highly expressed in testis (PHET), is overexpressed in SSc dermal fibroblasts and targeted by autoantibodies. PHET was identified by screening of HepG2 cDNA library using an SSc serum and was found to belong to UniGene cluster of sperm associated antigen 9 (SPAG9) from its nucleotide sequence. PHET mRNA expression was examined by RT-PCR using mRNA panels of human tissues, and PHET mRNA was highly expressed only in testis in normal tissues. Anti-PHET antibodies were detected in 8.4% of sera of SSc patients by immunoblotting, and associated with diffuse scleroderma and lung involvement. Expression of PHET mRNA and protein was increased in cultured SSc dermal fibroblasts compared with control fibroblasts. These results suggest that ectopic expression of PHET in dermal fibroblasts induces autoantibody production against PHET in patients with SSc.
AB - Systemic sclerosis (SSc) is characterized by excessive fibrosis and autoantibody production. However, the pathogenesis of SSc is still under investigation. We have demonstrated that a novel testicular antigen, protein highly expressed in testis (PHET), is overexpressed in SSc dermal fibroblasts and targeted by autoantibodies. PHET was identified by screening of HepG2 cDNA library using an SSc serum and was found to belong to UniGene cluster of sperm associated antigen 9 (SPAG9) from its nucleotide sequence. PHET mRNA expression was examined by RT-PCR using mRNA panels of human tissues, and PHET mRNA was highly expressed only in testis in normal tissues. Anti-PHET antibodies were detected in 8.4% of sera of SSc patients by immunoblotting, and associated with diffuse scleroderma and lung involvement. Expression of PHET mRNA and protein was increased in cultured SSc dermal fibroblasts compared with control fibroblasts. These results suggest that ectopic expression of PHET in dermal fibroblasts induces autoantibody production against PHET in patients with SSc.
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U2 - 10.1016/j.autrev.2006.08.006
DO - 10.1016/j.autrev.2006.08.006
M3 - Review article
C2 - 17317613
AN - SCOPUS:33847027591
SN - 1568-9972
VL - 6
SP - 228
EP - 231
JO - Autoimmunity Reviews
JF - Autoimmunity Reviews
IS - 4
ER -