Automated SNPs typing system based on the Invader assay

Hiroaki Nakahara; Naoya Hosono; Tetsushi Kitayama; Kazumasa Sekiguchi; Michiaki Kubo; Atsushi Takahashi; Yusuke Nakamura; Yoshiaki Yamano; Kazushige Kai

doi:10.1016/j.legalmed.2009.02.020

Automated SNPs typing system based on the Invader assay

Hiroaki Nakahara, Naoya Hosono, Tetsushi Kitayama, Kazumasa Sekiguchi, Michiaki Kubo, Atsushi Takahashi, Yusuke Nakamura, Yoshiaki Yamano, Kazushige Kai

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Typing of single nucleotide polymorphisms (SNPs) has advantages in forensic DNA identification because SNPs are abundant in genomic DNA and are easily detected using an automated high-throughput system. In this study, we examined the effectiveness of an automated multiplex SNPs typing system based on the "Invader assay". Using this system, multiplex SNPs typing is completed in 40 min without any complex procedures. The typing results were confirmed by direct sequencing, and no inconsistencies were detected between the sequencing results and the typing results. The population data of 21 SNPs from 113 Japanese indicates that the matching probability is about 1.3 × 10^-9. In our experiment, all of 21 SNPs were correctly detected from degraded samples from which typing of short tandem repeat markers was difficult.

Original language	English
Pages (from-to)	S111-S114
Journal	Legal Medicine
Volume	11
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/j.legalmed.2009.02.020
Publication status	Published - 04-2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Issues, ethics and legal aspects

Access to Document

10.1016/j.legalmed.2009.02.020

Cite this

@article{d96a204f08f146b886f7e056bc9d0619,

title = "Automated SNPs typing system based on the Invader assay",

abstract = "Typing of single nucleotide polymorphisms (SNPs) has advantages in forensic DNA identification because SNPs are abundant in genomic DNA and are easily detected using an automated high-throughput system. In this study, we examined the effectiveness of an automated multiplex SNPs typing system based on the {"}Invader assay{"}. Using this system, multiplex SNPs typing is completed in 40 min without any complex procedures. The typing results were confirmed by direct sequencing, and no inconsistencies were detected between the sequencing results and the typing results. The population data of 21 SNPs from 113 Japanese indicates that the matching probability is about 1.3 × 10-9. In our experiment, all of 21 SNPs were correctly detected from degraded samples from which typing of short tandem repeat markers was difficult.",

author = "Hiroaki Nakahara and Naoya Hosono and Tetsushi Kitayama and Kazumasa Sekiguchi and Michiaki Kubo and Atsushi Takahashi and Yusuke Nakamura and Yoshiaki Yamano and Kazushige Kai",

year = "2009",

month = apr,

doi = "10.1016/j.legalmed.2009.02.020",

language = "English",

volume = "11",

pages = "S111--S114",

journal = "Legal Medicine",

issn = "1344-6223",

publisher = "Elsevier",

number = "SUPPL. 1",

}

TY - JOUR

T1 - Automated SNPs typing system based on the Invader assay

AU - Nakahara, Hiroaki

AU - Hosono, Naoya

AU - Kitayama, Tetsushi

AU - Sekiguchi, Kazumasa

AU - Kubo, Michiaki

AU - Takahashi, Atsushi

AU - Nakamura, Yusuke

AU - Yamano, Yoshiaki

AU - Kai, Kazushige

PY - 2009/4

Y1 - 2009/4

N2 - Typing of single nucleotide polymorphisms (SNPs) has advantages in forensic DNA identification because SNPs are abundant in genomic DNA and are easily detected using an automated high-throughput system. In this study, we examined the effectiveness of an automated multiplex SNPs typing system based on the "Invader assay". Using this system, multiplex SNPs typing is completed in 40 min without any complex procedures. The typing results were confirmed by direct sequencing, and no inconsistencies were detected between the sequencing results and the typing results. The population data of 21 SNPs from 113 Japanese indicates that the matching probability is about 1.3 × 10-9. In our experiment, all of 21 SNPs were correctly detected from degraded samples from which typing of short tandem repeat markers was difficult.

AB - Typing of single nucleotide polymorphisms (SNPs) has advantages in forensic DNA identification because SNPs are abundant in genomic DNA and are easily detected using an automated high-throughput system. In this study, we examined the effectiveness of an automated multiplex SNPs typing system based on the "Invader assay". Using this system, multiplex SNPs typing is completed in 40 min without any complex procedures. The typing results were confirmed by direct sequencing, and no inconsistencies were detected between the sequencing results and the typing results. The population data of 21 SNPs from 113 Japanese indicates that the matching probability is about 1.3 × 10-9. In our experiment, all of 21 SNPs were correctly detected from degraded samples from which typing of short tandem repeat markers was difficult.

UR - http://www.scopus.com/inward/record.url?scp=67349214628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349214628&partnerID=8YFLogxK

U2 - 10.1016/j.legalmed.2009.02.020

DO - 10.1016/j.legalmed.2009.02.020

M3 - Article

C2 - 19278885

AN - SCOPUS:67349214628

SN - 1344-6223

VL - 11

SP - S111-S114

JO - Legal Medicine

JF - Legal Medicine

IS - SUPPL. 1

ER -

Automated SNPs typing system based on the Invader assay

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this