TY - JOUR
T1 - Autophagy-disrupted LC3 abundance leads to death of supporting cells of human oocytes
AU - Kang, Woojin
AU - Ishida, Eri
AU - Yamatoya, Kenji
AU - Nakamura, Akihiro
AU - Miyado, Mami
AU - Miyamoto, Yoshitaka
AU - Iwai, Maki
AU - Tatsumi, Kuniko
AU - Saito, Takakazu
AU - Saito, Kazuki
AU - Kawano, Natsuko
AU - Hamatani, Toshio
AU - Umezawa, Akihiro
AU - Miyado, Kenji
AU - Saito, Hidekazu
N1 - Publisher Copyright:
© 2018 The Authors
PY - 2018/9
Y1 - 2018/9
N2 - Autophagic recycling of cell parts is generally termed as the opposite of cell death. Here, we explored the relation between cell death and autophagy by examining granulosa cell layers that control oocyte quality, which is important for the success of fertilization. Granulosa cell layers were collected from infertile women and morphologically divided into four types, viz., mature (MCCs), immature (ICCs), and dysmature cumulus cells (DCCs), and mural granulosa cells (MGCs). Microtubule-associated protein light chain 3 (LC3), which is involved in autophagosome formation, was expressed excessively in DCCs and MGCs, and their chromosomal DNA was highly fragmented. However, autophagy initiation was limited to MGCs, as indicated by the expression of membrane-bound LC3-II and autophagy-related protein 7 (ATG7), an enzyme that converts LC3-I to LC3-II. Although pro-LC3 was accumulated, autophagy was disabled in DCCs, resulting in cell death. Our results suggest the possibility that autophagy-independent accumulation of pro-LC3 proteins leads to the death of human granulosa cells surrounding the oocytes and presumably reduces oocyte quality and female fertility.
AB - Autophagic recycling of cell parts is generally termed as the opposite of cell death. Here, we explored the relation between cell death and autophagy by examining granulosa cell layers that control oocyte quality, which is important for the success of fertilization. Granulosa cell layers were collected from infertile women and morphologically divided into four types, viz., mature (MCCs), immature (ICCs), and dysmature cumulus cells (DCCs), and mural granulosa cells (MGCs). Microtubule-associated protein light chain 3 (LC3), which is involved in autophagosome formation, was expressed excessively in DCCs and MGCs, and their chromosomal DNA was highly fragmented. However, autophagy initiation was limited to MGCs, as indicated by the expression of membrane-bound LC3-II and autophagy-related protein 7 (ATG7), an enzyme that converts LC3-I to LC3-II. Although pro-LC3 was accumulated, autophagy was disabled in DCCs, resulting in cell death. Our results suggest the possibility that autophagy-independent accumulation of pro-LC3 proteins leads to the death of human granulosa cells surrounding the oocytes and presumably reduces oocyte quality and female fertility.
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U2 - 10.1016/j.bbrep.2018.08.002
DO - 10.1016/j.bbrep.2018.08.002
M3 - Article
AN - SCOPUS:85051638911
SN - 2405-5808
VL - 15
SP - 107
EP - 114
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
ER -