B7-H3 expression in gastric cancer: A novel molecular blood marker for detecting circulating tumor cells

Takaaki Arigami, Yoshikazu Uenosono, Munetsugu Hirata, Shigehiro Yanagita, Sumiya Ishigami, Shoji Natsugoe

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)


The clinical significance of B7-H3 expression in gastric cancer remains unclear, although the B7 ligand family plays a critical role in the T cell-mediated immune response. We therefore investigated B7-H3 expression as a blood marker of circulating tumor cells and determined correlations with tumor progression in patients with gastric cancer. B7-H3 expression in gastric cell lines was initially evaluated by immunocytochemistry. Furthermore, we used quantitative RT-PCR to assess B7-H3 mRNA expression in four cell lines and in 95 blood specimens from patients with gastric cancer, as well as in 21 samples of peripheral blood lymphocytes from healthy volunteers. B7-H3 expression in cell lines was identified by immunocytochemistry and quantitative RT-PCR. Blood specimens from patients with gastric cancer contained significantly more copies of B7-H3 mRNA than those from healthy volunteers without cancer (P<0.0001). Levels of B7-H3 expression significantly correlated with overall stage (P=0.013). The 5-year survival rate was significantly lower in patients with high B7-H3 expression than with low expression (P=0.02). Multivariate analysis demonstrated that B7-H3 expression was an independent prognostic factor (P=0.046). Our results indicate that B7-H3 appears to be a useful blood marker for predicting tumor progression in gastric cancer.

Original languageEnglish
Pages (from-to)1019-1024
Number of pages6
JournalCancer science
Issue number5
Publication statusPublished - 05-2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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