Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells

Shamima Islam, Ferdaus Hassan, Gantsetseg Tumurkhuu, Jargalsaikhan Dagvadorj, Naoki Koide, Yoshikazu Naiki, Isamu Mori, Tomoaki Yoshida, Takashi Yokochi

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125 Citations (Scopus)


Lipopolysaccharide (LPS) is a potent bone resorbing factor. The effect of LPS on osteoclast formation was examined by using murine RAW 264.7 macrophage cells. LPS-induced the formation of multinucleated giant cells (MGC) in RAW 264.7 cells 3 days after the exposure. MGCs were positive for tartrate-resistant acid phosphatase (TRAP) activity. Further, MGC formed resorption pits on calcium-phosphate thin film that is a substrate for osteoclasts. Therefore, LPS was suggested to induce osteoclast formation in RAW 264.7 cells. LPS-induced osteoclast formation was abolished by anti-tumor necrosis factor (TNF)-α antibody, but not antibodies to macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-κB ligand (RANKL). TNF-α might play a critical role in LPS-induced osteoclast formation in RAW 264.7 cells. Inhibitors of NF-κB and stress activated protein kinase (SAPK/JNK) prevented the LPS-induced osteoclast formation. The detailed mechanism of LPS-induced osteoclast formation is discussed.

Original languageEnglish
Pages (from-to)346-351
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 24-08-2007

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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