Basic study of retinal stem/progenitor cell separation from mouse iris tissue

Naoki Yamamoto, Atsuhiro Tanikawa, Masayuki Horiguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We described the possibility of retinal regeneration using a novel and efficient technique for culturing and separating retinal stem/progenitor cells from iris tissue. Immunohistochemical staining of adult agouti mouse iris tissue revealed the presence of nestin/low-affinity neurotrophin receptor p75 (p75NTR)-positive cells on the endothelium camerae anterioris side. Cultured mouse iris-derived cells contained little or no melanin and were found to be positive for nestin. Most nestin-positive cells were analyzed for the coexpression of p75NTR as a cell membrane protein. When the p75 NTR was used as a marker to sort the cells, we obtained a dense population of nestin-positive cells. Furthermore, the nestin/p75 NTR-positive cells were able to differentiate into neural retina cells. Thus, this culture and separation technique is useful for obtaining retinal stem/progenitor cells from adult mouse iris tissue and for the efficient production of neural retina cells.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalMedical Molecular Morphology
Volume43
Issue number3
DOIs
Publication statusPublished - 01-09-2010

Fingerprint

Cell Separation
Iris
Nestin
Stem Cells
Retina
Nerve Growth Factor Receptor
Culture Techniques
Melanins
Endothelium
Regeneration
Membrane Proteins
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology

Cite this

Yamamoto, Naoki ; Tanikawa, Atsuhiro ; Horiguchi, Masayuki. / Basic study of retinal stem/progenitor cell separation from mouse iris tissue. In: Medical Molecular Morphology. 2010 ; Vol. 43, No. 3. pp. 139-144.
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Basic study of retinal stem/progenitor cell separation from mouse iris tissue. / Yamamoto, Naoki; Tanikawa, Atsuhiro; Horiguchi, Masayuki.

In: Medical Molecular Morphology, Vol. 43, No. 3, 01.09.2010, p. 139-144.

Research output: Contribution to journalArticle

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