Behavioral changes induced by stressful situations: Effects of enkephalins, dynorphin, and their interactions

A. Katoh, Toshitaka Nabeshima, T. Kameyama

Research output: Contribution to journalArticle

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Abstract

Effects of opioid peptides on behavioral changes induced by stressful situations were investigated. [D-Ala2,Met5]-enkephalinamide and [D-Ala2,D-Leu5]-enkephalin, degrading-enzyme-resistant derivatives of methionine-enkephalin and leucine-enkephalin, respectively, significantly attenuated both environment-induced conditioned suppression of motility and forced swimming-induced immobility in a dose-dependent manner. The [D-Ala2,Met5]-enkephalinamide- and [D-Ala2,D-Leu5]-enkephalin-induced attenuations were antagonized by naloxone at different doses. In contrast, dynorphin A(1-17) and [N-methyl-Tyr1, N-methyl-Arg7, D-Leu8]-dynorphin A(1-8) ethylamide (E 2078), a degrading-enzyme-resistant derivative of dynorpin, significantly potentiated both behavioral changes. The dynorphin- and E 2078-induced potentiations were inhibited by Mr 2266. [D-Ala2,Met5]-enkephalinamide-induced attenuation of environment-induced conditioned suppression of motility and forced swimming-induced immobility was prevented by treatment with dynorphin and E 2078 at the doses that did not affect the behavioral changes. This antagonism by both dynorphin and E 2078 was inhibited by Mr 2266. However, [D-Ala2,D-Leu5]-enkephalin-induced attenuation of both behavioral changes was not affected by either dynorphin or E 2078. These results indicated that activation of the methionine- or leucine-enkephalinergic system attenuates behavioral changes induced by stress, whereas activation of the dynorphinergic system potentiates those changes. Furthermore, the dynorphinergic system may interact with the methionine-enkephalinergic system, but not with the leucine-enkephalinergic system.

Original languageEnglish
Pages (from-to)600-607
Number of pages8
JournalJournal of Pharmacology and Experimental Therapeutics
Volume253
Issue number2
Publication statusPublished - 01-01-1990
Externally publishedYes

Fingerprint

Dynorphins
Enkephalins
dynorphin (1-8)
Leucine
Methionine
Leucine Enkephalin
Methionine Enkephalin
Opioid Peptides
Naloxone
E 2078
Enzymes
Ala(2)-enkephalinamide-met

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

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title = "Behavioral changes induced by stressful situations: Effects of enkephalins, dynorphin, and their interactions",
abstract = "Effects of opioid peptides on behavioral changes induced by stressful situations were investigated. [D-Ala2,Met5]-enkephalinamide and [D-Ala2,D-Leu5]-enkephalin, degrading-enzyme-resistant derivatives of methionine-enkephalin and leucine-enkephalin, respectively, significantly attenuated both environment-induced conditioned suppression of motility and forced swimming-induced immobility in a dose-dependent manner. The [D-Ala2,Met5]-enkephalinamide- and [D-Ala2,D-Leu5]-enkephalin-induced attenuations were antagonized by naloxone at different doses. In contrast, dynorphin A(1-17) and [N-methyl-Tyr1, N-methyl-Arg7, D-Leu8]-dynorphin A(1-8) ethylamide (E 2078), a degrading-enzyme-resistant derivative of dynorpin, significantly potentiated both behavioral changes. The dynorphin- and E 2078-induced potentiations were inhibited by Mr 2266. [D-Ala2,Met5]-enkephalinamide-induced attenuation of environment-induced conditioned suppression of motility and forced swimming-induced immobility was prevented by treatment with dynorphin and E 2078 at the doses that did not affect the behavioral changes. This antagonism by both dynorphin and E 2078 was inhibited by Mr 2266. However, [D-Ala2,D-Leu5]-enkephalin-induced attenuation of both behavioral changes was not affected by either dynorphin or E 2078. These results indicated that activation of the methionine- or leucine-enkephalinergic system attenuates behavioral changes induced by stress, whereas activation of the dynorphinergic system potentiates those changes. Furthermore, the dynorphinergic system may interact with the methionine-enkephalinergic system, but not with the leucine-enkephalinergic system.",
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Behavioral changes induced by stressful situations : Effects of enkephalins, dynorphin, and their interactions. / Katoh, A.; Nabeshima, Toshitaka; Kameyama, T.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 253, No. 2, 01.01.1990, p. 600-607.

Research output: Contribution to journalArticle

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T2 - Effects of enkephalins, dynorphin, and their interactions

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AB - Effects of opioid peptides on behavioral changes induced by stressful situations were investigated. [D-Ala2,Met5]-enkephalinamide and [D-Ala2,D-Leu5]-enkephalin, degrading-enzyme-resistant derivatives of methionine-enkephalin and leucine-enkephalin, respectively, significantly attenuated both environment-induced conditioned suppression of motility and forced swimming-induced immobility in a dose-dependent manner. The [D-Ala2,Met5]-enkephalinamide- and [D-Ala2,D-Leu5]-enkephalin-induced attenuations were antagonized by naloxone at different doses. In contrast, dynorphin A(1-17) and [N-methyl-Tyr1, N-methyl-Arg7, D-Leu8]-dynorphin A(1-8) ethylamide (E 2078), a degrading-enzyme-resistant derivative of dynorpin, significantly potentiated both behavioral changes. The dynorphin- and E 2078-induced potentiations were inhibited by Mr 2266. [D-Ala2,Met5]-enkephalinamide-induced attenuation of environment-induced conditioned suppression of motility and forced swimming-induced immobility was prevented by treatment with dynorphin and E 2078 at the doses that did not affect the behavioral changes. This antagonism by both dynorphin and E 2078 was inhibited by Mr 2266. However, [D-Ala2,D-Leu5]-enkephalin-induced attenuation of both behavioral changes was not affected by either dynorphin or E 2078. These results indicated that activation of the methionine- or leucine-enkephalinergic system attenuates behavioral changes induced by stress, whereas activation of the dynorphinergic system potentiates those changes. Furthermore, the dynorphinergic system may interact with the methionine-enkephalinergic system, but not with the leucine-enkephalinergic system.

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