Behavioural adaptations to addictive drugs in mice lacking the NMDA receptor ε1 subunit

Yoshiaki Miyamoto, Kiyofumi Yamada, Taku Nagai, Hisashi Mori, Masayoshi Mishina, Hiroshi Furukawa, Yukihiro Noda, Toshitaka Nabeshima

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59 Citations (Scopus)


N-methyl-D-aspartate (NMDA) receptors, a subtype of glutamate receptors (GluRs) formed by assembly of the GluRζ subunit (called NR1 in rats) with any one of four GluRε subunits (GluRε1-4; NR2A-D), play an important role in excitatory neurotransmission, synaptic plasticity and brain development. Recent pharmacological studies have also indicated a role for NMDA receptors in drug addiction. In the present study, we investigated the behavioural adaptations to addictive drugs such as phencyclidine (PCP), methamphetamine (MAP) and morphine (MOR) in mice lacking the GluRε1 subunit of the NMDA receptor. GluRε1 mutant mice exhibited a malfunction of NMDA receptors, as evidenced by the reduction of [3H]MK-801 binding in an autoradiographic receptor binding assay. GluRε1 mutant mice showed an attenuation of acute PCP- and MAP-induced hyperlocomotion. The development of sensitization by repeated treatment with PCP and MAP at a low, but not high, dose was also suppressed. The development of MOR-induced analgesic tolerance and naloxone-precipitated MOR withdrawal symptoms were attenuated in GluRε1 mutant mice. In the place conditioning test, PCP-induced place aversion in naive mice and place preference in PCP-pretreated mice, as well as MOR-induced place preference, were diminished whereas MAP-induced place preference was not affected in GluRε1 mutant mice. These findings provide genetic evidence that GluRε1 subunit-containing NMDA receptors are involved in certain aspects of drug addiction.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalEuropean Journal of Neuroscience
Issue number1
Publication statusPublished - 01-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)


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