TY - JOUR
T1 - Biochemical and crystallographic characterization of a Rho effector domain of the protein serine/threonine kinase N in a complex with RhoA
AU - Maesaki, Ryoko
AU - Shimizu, Toshiyuki
AU - Ihara, Kentaro
AU - Kuroda, Shinya
AU - Kaibuchi, Kozo
AU - Hakoshima, Toshio
N1 - Funding Information:
We thank J. Tsukamoto for her technical support in performing MALDI-TOF MS and N-terminal analysis. This work was supported by Grants in Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan to T.H. (10179104, 10359003, 09308025). K.I. was supported by a research fellowship for Young Scientists from JSPS.
PY - 1999/6/15
Y1 - 1999/6/15
N2 - The effector domain of human protein serine/threonine kinase N (PKN), an effector protein for the small GTP-binding protein Rho, was expressed and purified for protein characterization and crystallization, in a complex form with human RhoA. In solution, RhoA binds to the PKN effector domain with 1:2 stoichiometry in a GTP-dependent manner. The obtained complex crystals diffract to 2.2 Å resolution.
AB - The effector domain of human protein serine/threonine kinase N (PKN), an effector protein for the small GTP-binding protein Rho, was expressed and purified for protein characterization and crystallization, in a complex form with human RhoA. In solution, RhoA binds to the PKN effector domain with 1:2 stoichiometry in a GTP-dependent manner. The obtained complex crystals diffract to 2.2 Å resolution.
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U2 - 10.1006/jsbi.1999.4114
DO - 10.1006/jsbi.1999.4114
M3 - Article
C2 - 10388627
AN - SCOPUS:0033563754
VL - 126
SP - 166
EP - 170
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 2
ER -