TY - JOUR
T1 - Biochemical and crystallographic characterization of a Rho effector domain of the protein serine/threonine kinase N in a complex with RhoA
AU - Maesaki, Ryoko
AU - Shimizu, Toshiyuki
AU - Ihara, Kentaro
AU - Kuroda, Shinya
AU - Kaibuchi, Kozo
AU - Hakoshima, Toshio
PY - 1999/6/15
Y1 - 1999/6/15
N2 - The effector domain of human protein serine/threonine kinase N (PKN), an effector protein for the small GTP-binding protein Rho, was expressed and purified for protein characterization and crystallization, in a complex form with human RhoA. In solution, RhoA binds to the PKN effector domain with 1:2 stoichiometry in a GTP-dependent manner. The obtained complex crystals diffract to 2.2 Å resolution.
AB - The effector domain of human protein serine/threonine kinase N (PKN), an effector protein for the small GTP-binding protein Rho, was expressed and purified for protein characterization and crystallization, in a complex form with human RhoA. In solution, RhoA binds to the PKN effector domain with 1:2 stoichiometry in a GTP-dependent manner. The obtained complex crystals diffract to 2.2 Å resolution.
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U2 - 10.1006/jsbi.1999.4114
DO - 10.1006/jsbi.1999.4114
M3 - Article
C2 - 10388627
AN - SCOPUS:0033563754
VL - 126
SP - 166
EP - 170
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 2
ER -