Biochemical and crystallographic characterization of a Rho effector domain of the protein serine/threonine kinase N in a complex with RhoA

Ryoko Maesaki, Toshiyuki Shimizu, Kentaro Ihara, Shinya Kuroda, Kozo Kaibuchi, Toshio Hakoshima

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The effector domain of human protein serine/threonine kinase N (PKN), an effector protein for the small GTP-binding protein Rho, was expressed and purified for protein characterization and crystallization, in a complex form with human RhoA. In solution, RhoA binds to the PKN effector domain with 1:2 stoichiometry in a GTP-dependent manner. The obtained complex crystals diffract to 2.2 Å resolution.

Original languageEnglish
Pages (from-to)166-170
Number of pages5
JournalJournal of Structural Biology
Volume126
Issue number2
DOIs
Publication statusPublished - 15-06-1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology

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